单核细胞衍生的成纤维细胞生长因子与肿瘤坏死因子的物理化学比较

A physico-chemical comparison of the monocyte-derived fibroblast growth factor and the tumour necrosis factor.

作者信息

Austgulen R, Nissen-Meyer J

机构信息

Cell Research Laboratory, University of Trondheim, Norway.

出版信息

APMIS. 1988 Apr;96(4):352-6. doi: 10.1111/j.1699-0463.1988.tb05315.x.

DOI:10.1111/j.1699-0463.1988.tb05315.x

PMID:3370158

Abstract

The chromatographic characteristics of the human monocyte-derived growth stimulatory activity towards diploid FS-4 human fibroblasts has been studied. The fibroblast growth stimulatory factor had an apparent isoelectric point of 5.8 as determined by chromatofocusing, and a molecular weight in the range 70,000-30,000 as determined by gel filtration. The growth stimulatory factor eluted together with the tumour necrosis factor (TNF) upon cation exchange chromatography, chromatofocusing and gel filtration. Moreover, the fibroblast growth stimulatory activity in crude monocyte supernatants as well as the activity in the gel filtration column fractions was neutralized by antiserum raised against recombinant TNF (rTNF). The results indicate that the monocyte-derived fibroblast growth stimulatory activity is largely due to TNF.

摘要

对人单核细胞衍生的针对二倍体FS - 4人成纤维细胞的生长刺激活性的色谱特性进行了研究。通过色谱聚焦测定，成纤维细胞生长刺激因子的表观等电点为5.8，通过凝胶过滤测定，其分子量在70,000 - 30,000范围内。在阳离子交换色谱、色谱聚焦和凝胶过滤中，生长刺激因子与肿瘤坏死因子（TNF）一起洗脱。此外，粗单核细胞上清液中的成纤维细胞生长刺激活性以及凝胶过滤柱级分中的活性被抗重组TNF（rTNF）产生的抗血清中和。结果表明，单核细胞衍生的成纤维细胞生长刺激活性很大程度上归因于TNF。