Austgulen R, Hammerstrøm J, Nissen-Meyer J
J Leukoc Biol. 1987 Jul;42(1):1-8. doi: 10.1002/jlb.42.1.1.
Human monocytes cultured in vitro released factors with growth stimulatory effect upon human dermal fibroblasts. Monocytes in RPMI 1640 released a growth enhancing activity in nearly constant amounts during in vitro differentiation to macrophage-like cells. The growth stimulatory effect mediated by supernatants was reduced when lipopolysaccharide (LPS) or opsonized zymosan particles (OpZ) were added to monocytes during the first 5 days of in vitro culture, thereafter reaching similar levels. The release of interleukin 1 (IL-1) from monocytes was restricted to the first 2 days of culture. IL-1 production by unstimulated monocytes was negligible, while LPS induced a high release of IL-1 from monocytes. A rabbit antibody against human IL-1 abolished the IL-1 activity of the monocyte supernatants as assessed in a mouse thymocyte proliferation assay, but caused only a small reduction of the fibroblast proliferation activity. Thus, the fibroblast growth stimulatory activity mediated by monocytes was caused by factor(s) in addition to IL-1.
体外培养的人单核细胞释放出对人皮肤成纤维细胞具有生长刺激作用的因子。在RPMI 1640培养基中的单核细胞在体外分化为巨噬细胞样细胞的过程中，几乎以恒定的量释放出一种生长增强活性物质。当在体外培养的前5天向单核细胞中加入脂多糖（LPS）或调理酵母聚糖颗粒（OpZ）时，上清液介导的生长刺激作用会降低，此后达到相似水平。单核细胞中白细胞介素1（IL-1）的释放仅限于培养的前2天。未受刺激的单核细胞产生的IL-1可以忽略不计，而LPS可诱导单核细胞大量释放IL-1。在小鼠胸腺细胞增殖试验中评估发现，一种抗人IL-1的兔抗体消除了单核细胞上清液中的IL-1活性，但仅使成纤维细胞增殖活性略有降低。因此，单核细胞介导的成纤维细胞生长刺激活性是由除IL-1之外的其他因子引起的。
