Gioanni J, Farges M F, Duplay H, Hery M, Zanghellini E, Schneider M, Mazeau C, Namer M, Courdi A
Centre A.-Lacassagne, Nice, France.
Bull Cancer. 1988;75(3):285-90.
The clonogenicity in soft agar and the labeling index (LI) which represents the percentage of cells in the DNA synthesis phase, were studied in 59 breast cancer patients and these parameters were related to other known clinicopathological features, namely age, histological grading, estrogen and progesterone receptors and the status of axillary lymph nodes. Out of 59 tumors, 49 could be successfully cloned in soft agar and the mean plating efficiency (PE) was 0.1%. Low grade tumors were more frequently encountered in tumors which did not form colonies (P = 0.025). Cloned tumors had a higher mean LI (P = 0.05). A high PE was associated with low estrogen receptors (ER) (P = 0.03). Clonogenicity was not related to patient age, progesterone receptors (PR) or the status of axillary lymph nodes. These results suggest that a successful in vitro cloning and a high PE are associated with unfavorable prognostic factors in breast cancer.
对59例乳腺癌患者进行了软琼脂克隆形成能力及标记指数（LI，代表处于DNA合成期的细胞百分比）的研究，并将这些参数与其他已知的临床病理特征（即年龄、组织学分级、雌激素和孕激素受体以及腋窝淋巴结状态）相关联。在59个肿瘤中，49个能够在软琼脂中成功克隆，平均接种效率（PE）为0.1%。在未形成集落的肿瘤中，低级别肿瘤更为常见（P = 0.025）。克隆的肿瘤平均LI较高（P = 0.05）。高PE与低雌激素受体（ER）相关（P = 0.03）。克隆形成能力与患者年龄、孕激素受体（PR）或腋窝淋巴结状态无关。这些结果表明，体外成功克隆及高PE与乳腺癌不良预后因素相关。
