Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia.
Dis Aquat Organ. 2021 Mar 11;144:1-7. doi: 10.3354/dao03571.
The microsporidian parasite Enterocytozoon hepatopenaei (EHP) is an emerging problem in the marine shrimp industry, primarily in Asian countries such as China, Thailand, India, Malaysia, Indonesia, and Vietnam. A screening was conducted to investigate the prevalence of EHP after a fixed period of culturing for 1 rearing cycle in 3 states of Malaysia. The screening stages covered Penaeus vannamei post larvae (PL) and after 14-30, 31-50, 51-70, and 71-90 d of culture in 1 production cycle. A total of 279 samples were amplified using a PCR assay targeting the gene encoding a spore wall protein (SWP) of EHP. The EHP infection was initially detected in the hatchery and increased to 96.6% after the shrimp were transferred to the pond. The positive EHP sequence showed 91 to 100% similarity to sequences from India, Thailand, Vietnam, Indonesia, and Latin America. EHP infection increased throughout 1 rearing cycle due to factors such as the cannibalistic feeding habits of shrimp and the presence of unknown vectors or carriers of EHP in the culture ponds. Hence, the finding from the current study will be fundamental for other studies concerning EHP.
微孢子虫寄生虫肝肠胞虫（EHP）是海洋虾类养殖业中的一个新问题，主要发生在中国、泰国、印度、马来西亚、印度尼西亚和越南等亚洲国家。在马来西亚的 3 个州进行了为期 1 个养殖周期的固定培养后，进行了 EHP 流行情况的筛查。筛查阶段涵盖了凡纳滨对虾（PL）以及在 1 个生产周期中培养的 14-30、31-50、51-70 和 71-90 天的后虾。使用针对 EHP 孢子壁蛋白（SWP）基因的 PCR 检测对总共 279 个样本进行了扩增。EHP 感染最初在孵化场中检测到，当虾转移到池塘中后增加到 96.6%。阳性 EHP 序列与来自印度、泰国、越南、印度尼西亚和拉丁美洲的序列显示出 91%至 100%的相似性。由于虾的自相残杀的摄食习性以及池塘中 EHP 的未知载体或携带者的存在等因素，EHP 感染在整个养殖周期中不断增加。因此，本研究的结果将对其他有关 EHP 的研究具有重要意义。
