Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea.
Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea.
Biosci Biotechnol Biochem. 2021 Apr 24;85(5):1227-1234. doi: 10.1093/bbb/zbab018.
Among many factors of controlling stem cell differentiation, the key transcription factor upregulation via physical force is a good strategy on the lineage-specific differentiation of stem cells. The study aimed to compare growth and myogenic potentials between the parental cells (PCs) and the 1-day-old C2C12 spheroid-derived cells (SDCs) in two-dimensional (2D) and three-dimensional (3D) culture conditions through examination of the cell proliferation and the expression of myogenic genes. The data showed that 1-day-old spheroids had more intense expression of MyoD gene with respect to the PCs. The proliferation of the SDCs is significantly higher than the PCs in a time-dependent manner. The SDCs had also significantly higher myogenic potential than the PCs in 2D and 3D culture conditions. The results suggest that MyoD gene upregulation through cell-cell contacts is the good approach for preparation of seed cells in muscle tissue engineering.
在控制干细胞分化的众多因素中，通过物理力上调关键转录因子是一种针对干细胞谱系特异性分化的良好策略。本研究旨在通过检测细胞增殖和肌生成基因的表达，比较原代细胞 (PCs) 和 1 天龄 C2C12 球体衍生细胞 (SDCs) 在二维 (2D) 和三维 (3D) 培养条件下的生长和肌生成潜力。数据表明，1 天龄球体的 MyoD 基因表达强度高于 PCs。SDCs 的增殖速度明显高于 PCs，且呈时间依赖性。SDCs 在 2D 和 3D 培养条件下的肌生成潜力也明显高于 PCs。结果表明，通过细胞间接触上调 MyoD 基因是肌肉组织工程中制备种子细胞的良好方法。
