Lee Eun Ju, Shaikh Sibhghatulla, Lee Jin Hee, Hur Sun Jin, Choi Inho
Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 38541, South Korea.
Research Institute of Cell Culture, Yeungnam University, Gyeongsan, 38541, South Korea.
Sci Rep. 2025 Apr 24;15(1):14350. doi: 10.1038/s41598-025-98386-1.
Muscle satellite cells (MSCs) are the most commonly used cells in cultured meat research and development. Enhancing MSC proliferation and differentiation while reducing cell culture costs is requisite to commercializing cultured meat. This study explored the effects of Glycyrrhiza uralensis crude water extract (GU-CWE) and licochalcone A and B (Lic A or B) on the proliferation and differentiation of chicken, bovine, and porcine MSCs. While GU-CWE and Lic A and B had negligible effects on bovine and porcine MSCs, GU-CWE significantly enhanced chicken MSC differentiation, and Lic A and B promoted both the proliferation and differentiation of chicken MSCs. Furthermore, GU-CWE was found to mitigate reactive oxygen species activity during chicken MSC differentiation and promote cell proliferation and adhesion in spheroid culture, thereby maintaining a spherical shape. Collectively, this study suggests that GU-CWE and Lic A and B can significantly reduce costs and safely increase the productivity of chicken MSCs in cultured meat production processes.
肌肉卫星细胞（MSCs）是 cultured meat 研发中最常用的细胞。提高MSCs的增殖和分化能力，同时降低细胞培养成本，是 cultured meat 商业化的必要条件。本研究探讨了甘草粗水提取物（GU-CWE）和光甘草定A和B（Lic A或B）对鸡、牛和猪MSCs增殖和分化的影响。虽然GU-CWE以及Lic A和B对牛和猪的MSCs影响可忽略不计，但GU-CWE显著增强了鸡MSCs的分化，而Lic A和B促进了鸡MSCs的增殖和分化。此外，研究发现GU-CWE可减轻鸡MSCs分化过程中的活性氧物种活性，并促进细胞在球体培养中的增殖和黏附，从而保持球形。总体而言，本研究表明，GU-CWE以及Lic A和B可以显著降低成本，并在 cultured meat 生产过程中安全地提高鸡MSCs的生产力。
