血管外渗参与的血管细胞和黑色素瘤细胞的成像和分离在血管外渗过程中。
Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis.
机构信息
Division of Medical Oncology, Department of Medicine, Duke University Medical Center, Durham, NC, USA.
Duke Cancer Institute, Duke University Medical Center, Durham, NC, USA.
出版信息
Methods Mol Biol. 2021;2265:417-425. doi: 10.1007/978-1-0716-1205-7_30.
Abstract
Cancer mortality rates are primarily a result of cancer metastasis. Recent advances in microscopy technology allow for the imaging of circulating tumor cells (CTCs) as they extravasate (exit) blood vessels, a key step in the metastasis process. Here, we describe the use of intravital microscopy techniques to image and isolate both extravasating melanoma CTCs and the extravasation-participating endothelial cells. These techniques can be used as a means to study cancer metastasis and as a screening tool for anticancer therapeutics.
摘要
癌症死亡率主要是癌症转移的结果。最近显微镜技术的进步使得可以在循环肿瘤细胞（CTC）从血管逸出（离开）时对其进行成像，这是转移过程中的关键步骤。在这里，我们描述了使用活体显微镜技术来成像和分离正在逸出的黑色素瘤 CTC 和参与逸出的内皮细胞。这些技术可用于研究癌症转移，也可作为抗癌治疗的筛选工具。
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