Division of Medical Oncology, Department of Medicine, Duke University Medical Center, Durham, NC, USA.
Duke Cancer Institute, Duke University Medical Center, Durham, NC, USA.
Methods Mol Biol. 2021;2265:417-425. doi: 10.1007/978-1-0716-1205-7_30.
Cancer mortality rates are primarily a result of cancer metastasis. Recent advances in microscopy technology allow for the imaging of circulating tumor cells (CTCs) as they extravasate (exit) blood vessels, a key step in the metastasis process. Here, we describe the use of intravital microscopy techniques to image and isolate both extravasating melanoma CTCs and the extravasation-participating endothelial cells. These techniques can be used as a means to study cancer metastasis and as a screening tool for anticancer therapeutics.
癌症死亡率主要是癌症转移的结果。最近显微镜技术的进步使得可以在循环肿瘤细胞（CTC）从血管逸出（离开）时对其进行成像，这是转移过程中的关键步骤。在这里，我们描述了使用活体显微镜技术来成像和分离正在逸出的黑色素瘤 CTC 和参与逸出的内皮细胞。这些技术可用于研究癌症转移，也可作为抗癌治疗的筛选工具。
