Heyder Christoph, Gloria-Maercker Eva, Hatzmann Wolfgang, Zaenker Kurt S, Dittmar Thomas
Institute of Immunology, University of Witten/Herdecke, Witten, Germany.
Contrib Microbiol. 2006;13:200-208. doi: 10.1159/000092974.
In cancer the blood-borne spread of tumor cells leads to the formation of secondary tumors at distant loci, whereby the extravasation of tumor cells is a prerequisite step during hematogenous metastasis. In regard to the fate of endothelial cells located at the site of tumor cell infiltration, tumor cell-endothelial interactions were analyzed using an in vitro real-time model. This model shows the complete sequence of the transmigration process and gave new insights into the complex and dynamic cell-cell and cell-matrix interactions which occur during tumor cell transmigration across the endothelial barrier. An in vitro real-time apoptosis assay permits the distinction between apoptotic cell death from necrotic cell death. This model indicates that transmigration of tumor cell clusters derived from the invasive human bladder carcinoma cell line T24 irreversibly damages the endothelial cells by inducing apoptosis at the site of tumor cell infiltration. It is postulated here that apoptosis induction facilitates the removal of detached endothelial cells, thereby forestalling a local inflammatory response which might be detrimental to extravasating tumor cells.
在癌症中，肿瘤细胞通过血液传播会在远处位点形成继发性肿瘤，其中肿瘤细胞的外渗是血行转移过程中的一个先决步骤。关于位于肿瘤细胞浸润部位的内皮细胞的命运，使用体外实时模型分析了肿瘤细胞与内皮细胞的相互作用。该模型展示了迁移过程的完整序列，并为肿瘤细胞穿过内皮屏障迁移期间发生的复杂且动态的细胞间和细胞与基质间的相互作用提供了新的见解。体外实时凋亡检测能够区分凋亡性细胞死亡和坏死性细胞死亡。该模型表明，源自侵袭性人膀胱癌细胞系T24的肿瘤细胞簇的迁移通过在肿瘤细胞浸润部位诱导凋亡而不可逆地损伤内皮细胞。在此推测，凋亡诱导有助于清除脱离的内皮细胞，从而防止可能对渗出的肿瘤细胞有害的局部炎症反应。
