Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC, Australia.
Methods Mol Biol. 2021;2265:543-555. doi: 10.1007/978-1-0716-1205-7_38.
Here we describe the application of mass cytometry to analyze tumor-infiltrating lymphocytes in human melanoma. Mass cytometry is the coupling of flow cytometry and mass spectrometry, which allows for the simultaneous measurement of 40+ cell parameters on a per cell basis. Heavy metal-labeled antibodies can bind to proteins (CD markers, transcription factors, cytokines) on the cell surface and in the cytoplasm/nucleus. As labeled cells pass through the CyTOF, the instrument detects the heavy metals. Combining these signals allows description of melanoma tumor-infiltrating lymphocytes at a greater depth than alternative phenotyping strategies and enables detailed analyses of a variety of cellular parameters, including immune cell lineage, activation status, and functional polarization.
在这里，我们描述了利用液质联用技术分析人类黑色素瘤中的肿瘤浸润淋巴细胞。液质联用技术是流式细胞术和质谱技术的结合，允许在单细胞水平上同时测量 40 多个细胞参数。重金属标记的抗体可以与细胞表面和细胞质/核内的蛋白质（CD 标志物、转录因子、细胞因子）结合。当标记的细胞通过 CyTOF 时，仪器会检测到重金属。结合这些信号可以比替代表型策略更深入地描述黑色素瘤肿瘤浸润淋巴细胞，并能够对各种细胞参数进行详细分析，包括免疫细胞谱系、激活状态和功能极化。
