The Center of Gastrointestinal and Minimally Invasive Surgery, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu 610031, China.
Emergency Department, Third Clinical Medical College, Peking University, Beijing 100191, China.
Aging (Albany NY). 2021 Mar 11;13(8):11043-11060. doi: 10.18632/aging.202714.
The aim of the present study was to evaluate the involvement of CD52 in adipocytes as well as to explore its effect on type 2 diabetes mellitus (T2DM), and to improve our understanding of the potential molecular events of obesity with type 2 diabetes. Global changes in the CD52 expression patterns were detected in adipocytes and preadipocytes derived from obese and lean individuals. In particular, CD52 was identified as significantly differentially upregulated and was analyzed, both and , using various approaches. experiments, CD52 was a significantly up-regulated mRNA in mature adipocytes and preadipocytes. In addition, CD52 gradually increased with the differentiation of preadipocytes. experiments, the expression of CD52 in high-fat diet (HFD) -fed mice tended to be higher than that in regular diet (RD) -fed mice. Further analysis showed that CD52 expression was positively correlated with Smad3 and TGF-β in mice, and the downregulation of CD52 was accompanied by increased glucose tolerance and insulin sensitivity. Moreover, a comparison of CD4+CD52 T cells and CD4+CD52 T cells showed that many T2DM-related genes were aberrantly expressed. Overall, CD52 may functioned as an important potential target for obesity with T2DM via TGF-β/Smad3 axis.
本研究旨在评估 CD52 在脂肪细胞中的作用及其对 2 型糖尿病（T2DM）的影响，以期深入了解肥胖伴 2 型糖尿病的潜在分子事件。在肥胖和非肥胖个体来源的脂肪细胞和前体细胞中检测到 CD52 表达模式的全局变化。特别是，鉴定出 CD52 显著上调，并使用多种方法分析了 CD52 。实验表明，CD52 在成熟脂肪细胞和前体细胞中是显著上调的 mRNA。此外，CD52 在前体细胞分化过程中逐渐增加。实验表明，高脂肪饮食（HFD）喂养的小鼠中 CD52 的表达趋于高于正常饮食（RD）喂养的小鼠。进一步分析表明，CD52 的表达与小鼠中的 Smad3 和 TGF-β 呈正相关，CD52 的下调伴随着葡萄糖耐量和胰岛素敏感性的增加。此外，对 CD4+CD52 T 细胞和 CD4+CD52 T 细胞的比较表明，许多与 2 型糖尿病相关的基因表达异常。总的来说，CD52 可能通过 TGF-β/Smad3 轴成为肥胖伴 2 型糖尿病的一个重要潜在治疗靶点。
