Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China; Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, PR China.
Bioorg Med Chem Lett. 2021 May 15;40:127913. doi: 10.1016/j.bmcl.2021.127913. Epub 2021 Mar 9.
Resveratrol is a natural polyhydroxy trans-stilbene product with many biological activities. One of the most striking biological activities of it is its anti-aging potential. Resveratrol can exhibit anti-aging activity via a variety of signaling pathways, however, the repair effect of it on kidney and brain injury in aging mice induced by d-galactose and its regulation on klotho gene expression have not been reported. Herein, the anti-aging activity of resveratrol and its effect on the repair of kidney and brain injuries in d-galactose-induced aging mice, as well as its regulation of klotho gene expression in these two tissues were investigated. The results indicated that resveratrol could significantly increase the aged cell viability and improve the pathological status of aging mice via inhibiting the formation of malondialdehyde and enhancing the activities of superoxide dismutase and catalase. The histological analysis suggested that resveratrol could remarkably repair the damages of kidney and brain tissues in aging mice. Moreover, PCR and western blot have shown that resveratrol could obviously increase the anti-aging klotho gene expression in the above tissues. The data in this paper further revealed and enriched the anti-aging mechanism of resveratrol, and the methods established in this study can be used as a tool to evaluate the anti-aging activity of drugs to a certain extent.
白藜芦醇是一种天然的多羟基反式二苯乙烯产物，具有许多生物活性。它最引人注目的生物活性之一是其抗衰老潜力。白藜芦醇可以通过多种信号通路发挥抗衰老活性，然而，它对 D-半乳糖诱导的衰老小鼠肾和脑损伤的修复作用及其对 klotho 基因表达的调节作用尚未见报道。本研究旨在探讨白藜芦醇的抗衰老活性及其对 D-半乳糖诱导的衰老小鼠肾和脑损伤的修复作用，以及对这两种组织中 klotho 基因表达的调节作用。结果表明，白藜芦醇通过抑制丙二醛的形成和提高超氧化物歧化酶和过氧化氢酶的活性，显著增加衰老细胞的活力，改善衰老小鼠的病理状态。组织学分析表明，白藜芦醇能显著修复衰老小鼠的肾和脑组织损伤。此外，PCR 和 Western blot 结果表明，白藜芦醇能明显增加上述组织中抗衰老 klotho 基因的表达。本文的数据进一步揭示和丰富了白藜芦醇的抗衰老机制，本研究建立的方法可以在一定程度上作为评估药物抗衰老活性的工具。
