Li Shiyu, Wang Tianmin, Wu Binning, Fu Wenyi, Xu Baojun, Pamuru Ramachandra Reddy, Kennett Mary, Vanamala Jairam K P, Reddivari Lavanya
Department of Food Science, Purdue University, West Lafayette, Indiana, USA.
Department of Plant Science, Penn State University, University Park, Pennsylvania, USA.
J Nutr Biochem. 2021 Jul;93:108616. doi: 10.1016/j.jnutbio.2021.108616. Epub 2021 Mar 8.
Ulcerative colitis (UC), a major form of inflammatory bowel disease (IBD), is on the rise worldwide. Approximately three million people suffer from IBD in the United States alone, but the current therapeutic options (e.g., corticosteroids) come with adverse side effects including reduced ability to fight infections. Thus, there is a critical need for developing effective, safe and evidence-based food products with anti-inflammatory activity. This study evaluated the antiinflammatory potential of purple-fleshed potato using a dextran sodium sulfate (DSS) murine model of colitis. Mice were randomly assigned to control (AIN-93G diet), P15 (15% purple-fleshed potato diet) and P25 (25% purple-fleshed potato diet) groups. Colitis was induced by 2% DSS administration in drinking water for six days. The results indicated that purple-fleshed potato supplementation suppressed the DSS-induced reduction in body weight and colon length as well as the increase in spleen and liver weights. P15 and P25 diets suppressed the elevation in the intestinal permeability, colonic MPO activity, mRNA expression and protein levels of pro-inflammatory interleukins IL-6 and IL-17, the relative abundance of specific pathogenic bacteria such as Enterobacteriaceae, Escherichia coli (E. coli) and pks E. coli, and the increased flagellin levels induced by DSS treatment. P25 alone suppressed the elevated systemic MPO levels in DSS-exposed mice, and elevated the relative abundance of Akkermansia muciniphila (A. muciniphila) as well as attenuated colonic mRNA expression level of IL-17 and the protein levels of IL-6 and IL-1β. Therefore, the purple-fleshed potato has the potential to aid in the amelioration of UC symptoms.
溃疡性结肠炎（UC）是炎症性肠病（IBD）的一种主要形式，在全球范围内呈上升趋势。仅在美国，就有大约300万人患有IBD，但目前的治疗选择（如皮质类固醇）会带来不良副作用，包括抗感染能力下降。因此，迫切需要开发具有抗炎活性的有效、安全且有循证依据的食品。本研究使用葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型评估了紫肉马铃薯的抗炎潜力。将小鼠随机分为对照组（AIN-93G饮食）、P15组（15%紫肉马铃薯饮食）和P25组（25%紫肉马铃薯饮食）。通过在饮用水中给予2% DSS持续6天诱导结肠炎。结果表明，补充紫肉马铃薯可抑制DSS诱导的体重和结肠长度降低以及脾脏和肝脏重量增加。P15和P25饮食可抑制肠道通透性升高、结肠MPO活性、促炎白细胞介素IL-6和IL-17的mRNA表达及蛋白水平、特定病原菌（如肠杆菌科、大肠杆菌和产志贺毒素大肠杆菌）的相对丰度以及DSS处理诱导的鞭毛蛋白水平升高。单独的P25饮食可抑制DSS暴露小鼠全身MPO水平升高，提高嗜黏蛋白阿克曼氏菌（A. muciniphila）的相对丰度，并减弱结肠IL-17的mRNA表达水平以及IL-6和IL-1β的蛋白水平。因此，紫肉马铃薯具有改善UC症状的潜力。
