Chromatin Biology Laboratory, Institute of Cellular and Molecular Biology, Agricultural University of Georgia, Kakha Bendukidze Campus, 240 David Aghmashenebeli Alley, 0131, Tbilisi, Georgia.
Cellular Immunology Laboratory, Institute of Cellular and Molecular Biology, Agricultural University of Georgia, Kakha Bendukidze Campus, 240 David Aghmashenebeli Alley, 0131, Tbilisi, Georgia.
Biochem Biophys Res Commun. 2021 Apr 23;550:166-170. doi: 10.1016/j.bbrc.2021.03.005. Epub 2021 Mar 8.
In the present study the role of poly(ADP)ribosylation on rubitecan induced caspase dependent cell death was evaluated. We show that Top1 poisoning by rubitecan induces caspase mediated apoptosis which was reduced by PARP inhibitor olaparib in zebrafish embryo. Collectively our data introduces zebrafish as a valuable model for PARP related biomedical research.
在本研究中，评估了聚（ADP-核糖）化在鲁比替康诱导的半胱天冬酶依赖性细胞死亡中的作用。我们表明，鲁比替康引起的 Top1 中毒会诱导半胱天冬酶介导的细胞凋亡，而 PARP 抑制剂奥拉帕尼可减少斑马鱼胚胎中的这种凋亡。总之，我们的数据表明，斑马鱼是一种有价值的与 PARP 相关的生物医学研究模型。
