Cadete-Leite A, Alves M C, Tavares M A
Department of Anatomy, Porto School of Medicine, Portugal.
J Submicrosc Cytol Pathol. 1988 Jan;20(1):115-22.
Previous investigations have demonstrated that prolonged alcohol consumption induces alterations of the neuronal lysosomal system in the cerebellum and hippocampus. To assess the effects of chronic alcohol consumption and withdrawal in the adult rat prefrontal cortex, we studied the pyramidal cells in the prelimbic area taking into particular account their lysosomal components. Alcohol-fed groups for 6, 12 and 18 months, respective age-matched pair-fed controls, and a 6-month recovery group after 12 months of alcohol consumption were used. No quantitative changes of primary lysosomes were depicted. Transitional forms of these organelles towards lipofuscin granules were more numerous among alcohol-fed and recovery animals. A precocious and progressive accumulation of lipofuscin granules occurred in greater amounts in alcohol-fed animals, increasing with the age and remaining after 6-months of withdrawal. Multivesicular bodies increased in alcohol-fed and recovery animals, displaying an inverse progression relative to the lipofuscin deposition. These results show that chronic alcohol consumption and withdrawal enhance the dynamic potentialities of the cell lysosomal system.
先前的研究表明，长期饮酒会导致小脑和海马体中神经元溶酶体系统的改变。为了评估成年大鼠前额叶皮质中慢性饮酒和戒断的影响，我们研究了边缘前区的锥体细胞，并特别考虑了它们的溶酶体成分。使用了分别喂养6个月、12个月和18个月的酒精喂养组、各自年龄匹配的成对喂养对照组，以及饮酒12个月后的6个月恢复期组。未发现初级溶酶体的数量变化。在酒精喂养和恢复期动物中，这些细胞器向脂褐素颗粒转变的过渡形式更多。脂褐素颗粒在酒精喂养动物中更早且逐渐大量积累，随着年龄增长而增加，并在戒断6个月后仍存在。多泡体在酒精喂养和恢复期动物中增加，与脂褐素沉积呈相反趋势。这些结果表明，慢性饮酒和戒断增强了细胞溶酶体系统的动态潜能。
