Zhou Bo, Das Anirban, Zhong Muchun, Guo Qian, Zhang De-Wen, Hing Karin A, Sobrido Ana Jorge, Titirici Maria-Magdalena, Krause Steffi
School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
Biosens Bioelectron. 2021 May 15;180:113121. doi: 10.1016/j.bios.2021.113121. Epub 2021 Mar 2.
Photoelectrochemical imaging has great potential in the label-free investigation of cellular processes. Herein, we report a new fast photoelectrochemical imaging system (PEIS) for DC photocurrent imaging of live cells, which combines high speed with excellent lateral resolution and high photocurrent stability, which are all crucial for studying dynamic cellular processes. An analog micromirror was adopted to raster the sensor substrate, enabling high-speed imaging. α-FeO (hematite) thin films synthesized via electrodeposition were used as a robust substrate with high photocurrent and good spatial resolution. The capabilities of this system were demonstrated by monitoring cell responses to permeabilization with Triton X-100. The ability to carry out dynamic functional imaging of multiple cells simultaneously provides improved confidence in the data than could be achieved with the slower electrochemical single-cell imaging techniques described previously. When monitoring pH changes, the PEIS can achieve frame rates of 8 frames per second.
光电化学成像在细胞过程的无标记研究中具有巨大潜力。在此，我们报告一种用于活细胞直流光电流成像的新型快速光电化学成像系统（PEIS），它结合了高速、出色的横向分辨率和高光电流稳定性，这些对于研究动态细胞过程都至关重要。采用模拟微镜对传感器基板进行光栅扫描，实现高速成像。通过电沉积合成的α-FeO（赤铁矿）薄膜用作具有高光电流和良好空间分辨率的坚固基板。通过监测细胞对Triton X-100通透化的反应，证明了该系统的能力。与先前描述的较慢的电化学单细胞成像技术相比，同时对多个细胞进行动态功能成像的能力为数据提供了更高的可信度。在监测pH变化时，PEIS可以实现每秒8帧的帧率。
