Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, České Budějovice (Budweis), Czech Republic.
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Eur J Protistol. 2021 Jun;79:125778. doi: 10.1016/j.ejop.2021.125778. Epub 2021 Feb 2.
The expanding phylogenetic tree of trypanosomatid flagellates (Kinetoplastea: Trypanosomatidae) contains a long-known and phylogenetically well-supported species-rich lineage that was provisionally named as the 'jaculum' clade. Its members were found in representatives of several unrelated families of heteropteran bugs captured in South and Central America, Europe, Africa, and Asia. However, this group resisted introduction into the culture, a needed prerequisite for its proper characterization. Here we describe four new cultivable species, which parasitize various parts of their hosts' intestine, including the thoracic and abdominal part of the midgut, hindgut, and Malpighian tubules. Morphologically, the cultured flagellates vary from relatively short stumpy promastigotes to long slender leptomonad cells. Some species form straphangers (cyst-like amastigotes) both in vivo and in vitro, initially attached to the basal part of the flagellum of the mother cell, from which they subsequently detach. To formally classify this enigmatic monophyletic cosmopolitan clade, we erected Obscuromonas gen. nov., including five species: O. modryi sp. nov. (isolated from the true bug host species Riptortus linearis captured in the Philippines), O. volfi sp. nov. (from Catorhintha selector, Curaçao), O. eliasi sp. nov. (from Graptostethus servus, Papua New Guinea), O. oborniki sp. nov. (from Aspilocoryphus unimaculatus, Madagascar), and O. jaculum comb. nov. (from Nepa cinerea, France). Obscuromonas along with the genus Blastocrithidia belongs to the newly established Blastocrithidiinae subfam. nov.
动基体目（Kinetoplastea：Trypanosomatidae）的扩展系统发育树包含一个长期以来已知的、在系统发育上得到很好支持的、物种丰富的谱系，该谱系被暂时命名为“jaculum”群。其成员存在于中南美洲、欧洲、非洲和亚洲捕获的几个无关的异翅目臭虫代表中。然而，该群体拒绝被引入培养，这是对其进行适当描述的必要前提。在这里，我们描述了四个新的可培养物种，它们寄生在宿主肠道的不同部位，包括中肠的胸段和腹段、后肠和马氏管。从形态上看，培养的鞭毛虫从相对较短的短粗型前鞭毛体到长而细的动基体型细胞不等。一些物种在体内和体外形成链孢囊（囊状无鞭毛体），最初附着在母细胞鞭毛的基部，随后从那里脱落。为了正式分类这个神秘的单系世界性群，我们建立了 Obscuromonas 属，包括五个物种：O. modryi sp. nov.（从菲律宾捕获的真臭虫宿主物种 Riptortus linearis 中分离）、O. volfi sp. nov.（从 Catorhintha selector，Curaçao 中分离）、O. eliasi sp. nov.（从 Graptostethus servus，巴布亚新几内亚中分离）、O. oborniki sp. nov.（从 Madagascar 的 Aspilocoryphus unimaculatus 中分离）和 O. jaculum comb. nov.（从法国的 Nepa cinerea 中分离）。Obscuromonas 与 Blastocrithidia 属一起属于新成立的 Blastocrithidiinae 亚科。
