Dario Maria Augusta, Lisboa Cristiane Varella, Silva Marlon Vicente, Herrera Heitor Miraglia, Rocha Fabiana Lopes, Furtado Marina Carvalho, Moratelli Ricardo, Rodrigues Roque André Luiz, Jansen Ana Maria
Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil.
Programa de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil.
Int J Parasitol Parasites Wildl. 2021 Apr 20;15:58-69. doi: 10.1016/j.ijppaw.2021.04.003. eCollection 2021 Aug.
a monoxenous trypanosomatid considered restricted to insects, was recently reported to infect a bat. Herein, has been demonstrated to have a wider range of vertebrate hosts and distribution in Brazilian biomes than once thought. Parasites isolated from haemocultures were characterized using V7V8 SSU rDNA and glyceraldehyde 3-phosphate dehydrogenase genes. Coatis () in the Cerrado; marmosets ( sp.) and bats (, , ) in the Atlantic Forest; crab-eating foxes () and ocelot () in the Pantanal biomes were infected by trypanosomatids that displayed choanomastigote forms in haemoculture in Giemsa-stained slide smears. Molecular characterization and phylogenetic inference confirmed the infection of in these animals. Moreover, slight differences in sequences were observed. growth curves were counted at 27°C, 36°C and 37°C, and the morphotypes were able to grow and survive for up to 16 days. Serological titers for were observed in nonhuman primates, demonstrating that this parasite is able to induce a humoral immune response in an infected mammal. These results showed that host specificity in trypanosomatids is complex and far from understood.
一种曾被认为仅寄生于昆虫的单宿主锥虫目寄生虫，最近有报道称其可感染蝙蝠。在此研究中，已证明该寄生虫在巴西生物群落中的脊椎动物宿主范围和分布比之前认为的更广。从血培养物中分离出的寄生虫，利用V7V8小亚基核糖体DNA（SSU rDNA）和甘油醛-3-磷酸脱氢酶基因进行了特征鉴定。在塞拉多地区的南美浣熊（）；在大西洋森林中的狨猴（属）和蝙蝠（、、）；在潘塔纳尔生物群落中的食蟹狐（）和豹猫（）均被锥虫目寄生虫感染，这些寄生虫在吉姆萨染色涂片的血培养中呈现出鞭毛体形式。分子特征鉴定和系统发育推断证实了这些动物感染了。此外，还观察到序列存在细微差异。在27°C、36°C和37°C条件下对的生长曲线进行了计数，其形态型能够生长并存活长达16天。在非人类灵长类动物中观察到了针对的血清学滴度，表明这种寄生虫能够在受感染的哺乳动物中诱导体液免疫反应。这些结果表明，锥虫目寄生虫的宿主特异性很复杂，远未被了解。
