Pimenta P F, De Souza W
Departamento de Histologia e Embriologia, Universidade Federal do Rio de Janeiro, Brasil.
J Submicrosc Cytol Pathol. 1988 Jan;20(1):89-99.
The process of interaction between macrophages and promastigote and amastigote forms of Leishmania mexicana amazonensis was analyzed using freeze fracture and cytochemistry. The promastigotes inside endocytic vacuoles of macrophages presented an altered distribution of intramembranous particles and a wavy aspect of the plasma membrane. However, amastigotes did not show such alterations. The membrane alterations are probably caused by intracellular cell lysis of the promastigotes by the macrophages. An accumulation of intramembranous particles was seen in the plasma membrane of amastigote forms in the area of adhesion to the macrophages. The parasitophorous vacuole membrane had intramembranous particles randomly distributed. The enzyme activity of Mg++-ATPase, 5'-nucleotidase and NAD(P)H-oxidase was cytochemically detected, at the ultrastructural level, in normal mouse peritoneal macrophages and in macrophages infected with Leishmania mexicana amazonensis. Mg++-ATPase and 5'-nucleotidase are uniformly distributed throughout the macrophage's plasma membrane but were not detected in the membrane lining endocytic vacuoles containing ingested parasites (parasitophorous vacuole). NAD(P)H-oxidase activity was seen in those portions of the macrophage's plasma membrane which enter in direct contact with parasites and also in association with the membrane of the parasitophorous vacuole. The amount of reaction product, indicative of NAD(P)H-oxidase activity, was larger in macrophages which interacted with the promastigote than in those which interacted with the amastigote form of L. mexicana amazonensis. Concanavalin A binding sites and anionic sites of the macrophage's surface, labeled before the interaction, are not interiorized together with the parasites, however, are observed in endocytic vacuoles which do not contain parasites.
利用冷冻断裂和细胞化学方法分析了巨噬细胞与亚马逊利什曼原虫前鞭毛体和无鞭毛体形式之间的相互作用过程。巨噬细胞内吞泡中的前鞭毛体呈现出膜内颗粒分布改变以及质膜呈波浪状。然而，无鞭毛体未显示出此类改变。膜改变可能是由于巨噬细胞对前鞭毛体的细胞内裂解所致。在无鞭毛体与巨噬细胞黏附区域的质膜中可见膜内颗粒的积累。寄生泡膜的膜内颗粒随机分布。在超微结构水平上，通过细胞化学方法检测了正常小鼠腹腔巨噬细胞和感染亚马逊利什曼原虫的巨噬细胞中Mg++-ATP酶、5'-核苷酸酶和NAD(P)H氧化酶的酶活性。Mg++-ATP酶和5'-核苷酸酶均匀分布于巨噬细胞的整个质膜，但在含有摄入寄生虫的内吞泡内衬膜（寄生泡）中未检测到。NAD(P)H氧化酶活性见于巨噬细胞质膜与寄生虫直接接触的部分以及与寄生泡膜相关的部分。与前鞭毛体相互作用的巨噬细胞中，指示NAD(P)H氧化酶活性的反应产物量大于与亚马逊利什曼原虫无鞭毛体形式相互作用的巨噬细胞。在相互作用前标记的巨噬细胞表面的伴刀豆球蛋白A结合位点和阴离子位点，不会与寄生虫一起内化，然而，在不含寄生虫的内吞泡中可观察到。
