Shouval D, Anton M, Galun E, Sherwood J B
Department of Medicine A, Hadassah University Hospital, Jerusalem, Israel.
Cancer Res. 1988 Jun 15;48(12):3430-4.
An established cloned human renal carcinoma line RC-1, which has been continuously maintained in culture for several years and which produces erythropoietin, was injected s.c. into BALB/c athymic mice and produced tumors. Tumorigenicity was directly correlated with the number of RC-1 cells inoculated. Tumor cell histology resembled the original patient-derived tumor. Tumor-bearing mice developed hepatosplenomegaly and significant reticulocytosis with elevated hemoglobin and hematocrit values that were proportional to tumor mass. In addition, red cell mass and blood volume of nude mice increased over 100% as compared to control mice or to animals bearing nonrelevant neoplasms. Large amounts of immunoreactive erythropoietin could be extracted from the nude mouse RC-1 tumors. These results indicate that the RC-1 cell line is tumorigenic and produces biologically active erythropoietin in vivo in athymic mouse hosts, thus providing a reproducible model to study ectopic erythropoietin production and its regulation in vivo.
已建立的克隆人肾癌细胞系RC-1在培养中已连续维持数年且能产生促红细胞生成素，将其皮下注射到BALB/c无胸腺小鼠体内可产生肿瘤。致瘤性与接种的RC-1细胞数量直接相关。肿瘤细胞组织学与原始患者来源的肿瘤相似。荷瘤小鼠出现肝脾肿大和显著的网织红细胞增多，血红蛋白和血细胞比容值升高，且与肿瘤大小成正比。此外，与对照小鼠或荷无关肿瘤的动物相比，裸鼠的红细胞量和血容量增加超过100%。可从裸鼠的RC-1肿瘤中提取大量免疫反应性促红细胞生成素。这些结果表明，RC-1细胞系具有致瘤性，并在无胸腺小鼠宿主体内产生具有生物活性的促红细胞生成素，从而为研究体内异位促红细胞生成素的产生及其调节提供了一个可重复的模型。
