Naito S, von Eschenbach A C, Fidler I J
J Natl Cancer Inst. 1987 Feb;78(2):377-85.
This study was designed to determine the influence of implantation site on the growth of local "primary tumors" and metastatic behavior of human renal cell carcinoma (HRCC) cells. HRCC lines were established in vitro from a surgical specimen (SN12C) or from a liver metastasis produced in BALB/c nude mice by cells from the original specimen implanted into their kidney (SN12L1). Cultured HRCC cells of both lines were injected into the subcutis and the renal subcapsule (RSC) of nude mice. Tumor growth and metastatic behavior were monitored. In both cell lines, tumor cells injected into the RSC displayed faster growth and produced more systemic metastases than did cells injected sc. The kidney tumors were large, invasive, highly vascularized, nonencapsulated, and with minimal central necrosis. In contrast, the subcutaneous tumors were highly encapsulated, with peripheral vascularization and extensive necrosis that developed as early as 2 weeks after sc implantation. The SN12L1 cells were also implanted into nude mice by iv, ip, and intrasplenic injections. The most dramatic production of metastasis occurred after the tumor cells were injected into the kidney. These results indicate that the biologic behavior of this HRCC is influenced by the implantation site in nude mice. At least for the HRCC tested, the kidney is the natural organ for growth. Implantation into the RSC is advantageous for the study of the biology and therapy of HRCC in nude mice.
本研究旨在确定植入部位对人肾细胞癌（HRCC）细胞局部“原发性肿瘤”生长及转移行为的影响。HRCC细胞系由手术标本（SN12C）或通过将原标本的细胞植入BALB/c裸鼠肾脏所产生的肝转移灶（SN12L1）体外建立。将两株培养的HRCC细胞注射到裸鼠的皮下和肾被膜下（RSC）。监测肿瘤生长和转移行为。在两株细胞系中，注射到RSC的肿瘤细胞比皮下注射的细胞生长更快，产生的全身转移更多。肾肿瘤体积大、具有侵袭性、血管丰富、无包膜，且中央坏死极少。相比之下，皮下肿瘤包膜完整，有周边血管形成，且早在皮下植入后2周就出现广泛坏死。SN12L1细胞还通过静脉注射、腹腔注射和脾内注射植入裸鼠。肿瘤细胞注射到肾脏后发生的转移最为显著。这些结果表明，这种HRCC的生物学行为受裸鼠植入部位的影响。至少对于所测试的HRCC而言，肾脏是其生长的天然器官。植入到RSC有利于在裸鼠中研究HRCC的生物学特性和治疗方法。
