Instituto Biofisika UPV/EHU, CSIC, Barrio Sarriena s/n, Leioa E-48940, Bizkaia, Spain.
Departamento de Bioquímica y Biología Molecular, UPV/EHU, Barrio Sarriena s/n, Leioa E-48940, Bizkaia, Spain.
ACS Appl Mater Interfaces. 2021 Mar 24;13(11):12941-12949. doi: 10.1021/acsami.1c01291. Epub 2021 Mar 11.
Fluorinated nanoparticles have increasing applications, but they are still challenging to prepare, especially in the case of water-soluble fluorinated nanoparticles. Herein, a fluorine labeling strategy is presented that is based on the conjugation of custom-made small fluorinated building blocks, obtained by simple synthetic transformations, with carboxylated gold nanoparticles through a convenient phase-transfer process. The synthesis of four fluorinated building blocks with different chemical shifts in F nuclear magnetic resonance and varied functionalities is reported, along with their conjugation onto nanoparticles. Fluorinated nanoparticles of small core size obtained by this conjugation methodology and by direct synthesis presented high transverse relaxation times () ranging from 518 to 1030 ms, and a large number of equivalent fluorine atoms per nanoparticle (340-1260 fluorine atoms), which made them potential candidates for F magnetic resonance related applications. Finally, nontargeted fluorinated nanoparticles were probed by performing F magnetic resonance spectroscopy (F MRS) in mice. Nanoparticles were detected at both 1 and 2 h after being injected. F MRI images were also acquired after either intravenous or subcutaneous injection. Their fate was studied by analyzing the gold content in tissues by ICP-MS. Thus, the present work provides a general fluorination strategy for nanoparticles and shows the potential use of small fluorinated nanoparticles in magnetic-resonance-related applications.
氟纳米粒子的应用越来越广泛，但它们的制备仍然具有挑战性，特别是水溶性氟纳米粒子。在此，提出了一种基于定制的小氟化砌块的氟化标记策略，这些砌块是通过简单的合成转化获得的，并通过方便的相转移过程与羧基化金纳米粒子偶联。报道了四种具有不同化学位移和不同功能的氟核磁共振的氟化砌块的合成及其在纳米粒子上的偶联。通过这种偶联方法和直接合成获得的小核尺寸的氟化纳米粒子具有较高的横向弛豫时间（），范围为 518 至 1030 ms，并且每个纳米粒子上具有大量等效的氟原子（340-1260 个氟原子），这使它们成为 F 磁共振相关应用的潜在候选物。最后，通过在小鼠中进行 19 F 磁共振波谱（19 F MRS）探测非靶向氟化纳米粒子。在注射后 1 和 2 小时检测到纳米粒子。静脉内或皮下注射后也获得了 F MRI 图像。通过 ICP-MS 分析组织中的金含量来研究它们的命运。因此，本工作为纳米粒子提供了一种通用的氟化策略，并展示了小氟化纳米粒子在磁共振相关应用中的潜在用途。
