State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Fujian Provincial Key Laboratory of Chemical Biology, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Renji Medical Research Center, Chengdu Second People's Hospital, Chengdu 610011, China.
ACS Nano. 2023 Mar 14;17(5):5014-5024. doi: 10.1021/acsnano.2c12793. Epub 2023 Mar 2.
Fluorine-19 magnetic resonance imaging (F MRI) is gaining widespread interest from the fields of biomolecule detection, cell tracking, and diagnosis, benefiting from its negligible background, deep tissue penetration, and multispectral capacity. However, a wide range of F MRI probes are in great demand for the development of multispectral F MRI due to the limited number of high-performance F MRI probes. Herein, we report a type of water-soluble molecular F MRI nanoprobe by conjugating fluorine-containing moieties with a polyhedral oligomeric silsesquioxane (POSS) cluster for multispectral color-coded F MRI. These chemically precise fluorinated molecular clusters are of excellent aqueous solubility with relatively high F contents and of single F resonance frequency with suitable longitudinal and transverse relaxation times for high-performance F MRI. We construct three POSS-based molecular nanoprobes with distinct F chemical shifts at -71.91, -123.23, and -60.18 ppm and achieve interference-free multispectral color-coded F MRI of labeled cells and . Moreover, F MRI reveals that these molecular nanoprobes could selectively accumulate in tumors and undergo rapid renal clearance afterward, illustrating their favorable behavior for biomedical applications. This study provides an efficient strategy to expand the F probe libraries for multispectral F MRI in biomedical research.
氟-19 磁共振成像（F MRI）因其背景信号低、组织穿透深和多谱能力，在生物分子检测、细胞示踪和诊断等领域受到广泛关注。然而，由于高性能 F MRI 探针数量有限，开发多谱 F MRI 需要各种 F MRI 探针。在此，我们通过将含氟基团与多面体低聚倍半硅氧烷（POSS）簇缀合，报告了一种水溶性分子 F MRI 纳米探针，用于多谱彩色编码 F MRI。这些化学精确的氟化分子簇具有极好的水溶性，具有相对较高的 F 含量和合适的纵向和横向弛豫时间，单 F 共振频率适用于高性能 F MRI。我们构建了三种基于 POSS 的分子纳米探针，它们的 F 化学位移分别为-71.91、-123.23 和-60.18 ppm，实现了标记细胞和 的无干扰多谱彩色编码 F MRI。此外，F MRI 表明这些分子纳米探针可以选择性地在肿瘤中积累，随后迅速从肾脏清除，说明它们在生物医学应用中具有良好的 行为。这项研究为生物医学研究中多谱 F MRI 的 F 探针库的扩展提供了一种有效的策略。
