Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
School of Medicine, Ningbo University, Ningbo 315211, China.
Biointerphases. 2021 Jan 22;16(1):011003. doi: 10.1116/6.0000737.
Biofouling on material surfaces is a ubiquitous problem in a variety of fields. In aqueous environments, the process of biofouling initiates with the formation of a layer of macromolecules called the conditioning layer on the solid-liquid interface, followed by the adhesion and colonization of planktonic bacteria and the subsequent biofilm development and maturation. In this study, the extracellular polymeric substances (EPS) secreted by Bacillus subtilis were collected and used to prepare conditioning layers on inert surfaces. The morphologies and antifouling performances of the EPS conditioning layers were investigated. It was found that the initial adhesion of Escherichia coli was inhibited on the surfaces precoated with EPS conditioning layers. To further explore the underlying antifouling mechanisms of the EPS conditioning layers, the respective roles of two constituents of B. subtilis EPS (γ-polyglutamic acid and surfactin) were investigated. This study has provided the possibility of developing a novel interfacial antifouling additive with the advantages of easy preparation, nontoxicity, and environmental friendliness.
生物污垢在材料表面是一个普遍存在的问题，在各种领域中。在水的环境中，生物污垢的过程首先与形成的一层高分子物质称为调理层在固液界面，随后的粘附和浮游细菌的殖民化和随后的生物膜的发展和成熟。在这项研究中，枯草芽孢杆菌分泌的胞外聚合物（EPS）被收集并用于制备惰性表面的调理层。对 EPS 调理层的形态和防污性能进行了研究。结果发现，在预先涂有 EPS 调理层的表面上，大肠杆菌的初始粘附被抑制。为了进一步探讨 EPS 调理层的潜在防污机制，研究了枯草芽孢杆菌 EPS 的两种成分（γ-聚谷氨酸和表面活性剂）各自的作用。本研究为开发一种新型的界面防污添加剂提供了可能，该添加剂具有制备简单、无毒、环保等优点。
