Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, 450052, Henan, People's Republic of China.
Department of Thyroid Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, No.7 Weiwu Road, Zhengzhou, 450003, Henan, People's Republic of China.
J Physiol Sci. 2021 Mar 11;71(1):9. doi: 10.1186/s12576-021-00793-2.
Long noncoding RNAs (lncRNAs) play an essential role in the progression of papillary thyroid cancer (PTC). However, the expression and function of lncRNA cancer susceptibility candidate 7 (CASC7) in PTC remain unknown. The purpose of this study was to investigate the role and molecular mechanism of CASC7 in regulating PTC cell behavior. The expression of CASC7, miR-34a-5p, and tumor protein P73 (TP73) was determined by qRT-PCR and western blot. Cell proliferation was examined by MTT assay. Cell apoptosis was assessed by flow cytometry following Annexin V and PI staining. Cell migration was determined by Transwell migration assay. The interaction between miR-34a-5p and CASC7 or TP73 was examined by luciferase reporter assay. CASC7 and TP73 expression were significantly lower, whereas miR-34a-5p expression was higher in PTC tissues than the adjacent normal tissues. Furthermore, CASC7 overexpression inhibited cell proliferation and migration, whereas facilitated cell apoptosis in human PTC cell lines (K1 and TPC-1). Mechanistically, CASC7 acted as a sponge of miR-34a-5p to upregulate TP73 expression. Moreover, miR-34a-5p mimic transfection could abate the CASC7-regulated PTC cell proliferation, migration, and apoptosis. Collectively, CASC7 inhibited the proliferation and migration of PTC cells by sponging miR-34a-5p to upregulate TP73 expression.
长链非编码 RNA（lncRNA）在甲状腺乳头状癌（PTC）的进展中发挥着重要作用。然而，lncRNA 癌症易感性候选物 7（CASC7）在 PTC 中的表达和功能仍不清楚。本研究旨在探讨 CASC7 在调节 PTC 细胞行为中的作用和分子机制。通过 qRT-PCR 和 Western blot 测定 CASC7、miR-34a-5p 和肿瘤蛋白 P73（TP73）的表达。通过 MTT 测定法检测细胞增殖。通过 Annexin V 和 PI 染色后流式细胞术评估细胞凋亡。通过 Transwell 迁移测定法测定细胞迁移。通过荧光素酶报告测定法检测 miR-34a-5p 与 CASC7 或 TP73 的相互作用。与相邻正常组织相比，PTC 组织中 CASC7 和 TP73 的表达明显降低，而 miR-34a-5p 的表达则升高。此外，CASC7 过表达抑制人 PTC 细胞系（K1 和 TPC-1）中的细胞增殖和迁移，而促进细胞凋亡。从机制上讲，CASC7 作为 miR-34a-5p 的海绵体来上调 TP73 的表达。此外，miR-34a-5p 模拟转染可以减轻 CASC7 调节的 PTC 细胞增殖、迁移和凋亡。总之，CASC7 通过海绵 miR-34a-5p 来上调 TP73 的表达，从而抑制 PTC 细胞的增殖和迁移。
