维甲酸诱导耳蜗神经祖细胞向毛细胞分化。

Retinoic acid induces differentiation of cochlear neural progenitor cells into hair cells.

机构信息

The Second Affiliated Hospital of Fujian Medical University, Department of Otolaryngology, Fujian, China.

Fujian Medical University Union Hospital, Department of Otolaryngology, Fujian, China.

出版信息

Braz J Otorhinolaryngol. 2022 Nov-Dec;88(6):962-967. doi: 10.1016/j.bjorl.2021.01.005. Epub 2021 Feb 21.

DOI:10.1016/j.bjorl.2021.01.005

PMID:33707121

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9615533/

Abstract

INTRODUCTION

Inner ear progenitor cells have the potential for multi-directional differentiation. Retinoic acid is an important requirement for the development of the inner ear. Blocking the Curtyr's retinoic acid signaling pathway can significantly reduce the number of hair cells. Therefore, we believe that retinoic acid may induce the regeneration of inner ear hair cells.

OBJECTIVE

To investigate whether the cochlear neural progenitor cells maintain the characteristics of stem cells during recovery and subculture, whether retinoic acid can induce cochlear neural progenitor cells into hair cells in vitro, and whether retinoic acid promotes or inhibits the proliferation of cochlear neural progenitor cells during differentiation.

METHODS

Cochlear neural progenitor cells were cultured and induced in DMEM/F12+RA (10M) and then detected the expressions of hair cell markers (Math1 and MyosinVIIa) by immunofluorescence cytochemistry and realtime-polymerase chain reaction, and the proliferation of cochlear neural progenitor cells was detected by Brdu.

RESULTS

The nestin of cochlear neural progenitor cells was positively expressed. The ratios of Math1-positive cells in the control group and experimental group were 1.5% and 63%, respectively; the ratios of MyosinVIIa-positive cells in the control group and experimental group were 0.96% and 56%, respectively (p<0.05). The ratios of Brdu-labeled cells in retinoic acid group, group PBS, and group FBS were 20.6%, 29.9%, and 54.3%, respectively; however, the proliferation rate in the experimental group decreased.

CONCLUSION

Retinoic acid can promote cochlear neural progenitor cells to differentiate into the hair cells.

摘要

简介

内耳祖细胞具有多向分化的潜能。视黄酸是内耳发育的重要要求。阻断 Curtyr 的视黄酸信号通路可以显著减少毛细胞的数量。因此，我们认为视黄酸可能诱导内耳毛细胞的再生。

目的

探讨耳蜗神经祖细胞在恢复和亚培养过程中是否保持干细胞的特征，视黄酸是否能诱导耳蜗神经祖细胞体外分化为毛细胞，以及视黄酸在分化过程中是促进还是抑制耳蜗神经祖细胞的增殖。

方法

在 DMEM/F12+RA（10M）中培养和诱导耳蜗神经祖细胞，然后通过免疫荧光细胞化学和实时聚合酶链反应检测毛细胞标志物（Math1 和 MyosinVIIa）的表达，通过 Brdu 检测耳蜗神经祖细胞的增殖。

结果

耳蜗神经祖细胞巢蛋白呈阳性表达。对照组和实验组 Math1 阳性细胞的比例分别为 1.5%和 63%，MyosinVIIa 阳性细胞的比例分别为 0.96%和 56%（p<0.05）。视黄酸组、PBS 组和 FBS 组 Brdu 标记细胞的比例分别为 20.6%、29.9%和 54.3%，但实验组的增殖率下降。