Jiang Wei, Pan Rui, Buitrago Sebastian, Wu Chu, Abdelaziz Mohamad E, Oelmüller Ralf, Zhang Wenying
Hubei Collaborative Innovation Center for Grain Industry/Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025 China.
College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025 China.
Physiol Mol Biol Plants. 2021 Feb;27(2):189-201. doi: 10.1007/s12298-020-00922-y. Epub 2021 Feb 4.
Freezing stress is a serious environmental factor that obstructs plant development. The root endophytic fungus has proved to be effective to confer abiotic stress tolerance to host plants. To investigate how improves freezing tolerance, we compared the expression profiles of -colonized and uncolonized seedlings either exposed to freezing stress or not. Nearly 24 million (93.5%) reads were aligned on the genome. 634 genes were differentially expressed between colonized and uncolonized exposed to freezing stress. Interestingly, 193 genes did not respond to freezing stress but were up-regulated by under freezing stress. Freezing stress-responsive genes encoded various members of the WRKY, ERF, bHLH, HSF, MYB and NAC transcription factor families. The qRT-PCR analyses confirmed the high-throughput sequencing results for 28 genes. Functional enrichment analysis indicated that the fungus mainly controls genes for freezing-stress related proteins involved in lipid and ion transport, metabolism pathways and phytohormone signaling. Our findings identified novel target genes of in freezing-stress exposed plants and highlight the benefits of the endophyte for plants exposed to a less investigated environmental threat.
The online version of this article (10.1007/s12298-020-00922-y) contains supplementary material, which is available to authorized users.
冻害胁迫是阻碍植物生长发育的严重环境因素。事实证明，根部内生真菌能有效赋予宿主植物非生物胁迫耐受性。为了研究[内生真菌名称]如何提高植物的抗冻性，我们比较了经冻害胁迫处理和未经冻害胁迫处理的被[内生真菌名称]定殖和未被定殖的[植物名称]幼苗的基因表达谱。近2400万个（93.5%） reads比对到了[植物名称]基因组上。在遭受冻害胁迫的被定殖和未被定殖的[植物名称]之间，有634个基因差异表达。有趣的是，193个[植物名称]基因对冻害胁迫无响应，但在冻害胁迫下被[内生真菌名称]上调表达。冻害胁迫响应基因编码WRKY、ERF、bHLH、HSF、MYB和NAC转录因子家族的多个成员。qRT-PCR分析证实了28个基因的高通量测序结果。功能富集分析表明，该真菌主要调控与脂质和离子转运、代谢途径及植物激素信号传导相关的冻害胁迫相关蛋白的基因。我们的研究结果确定了在遭受冻害胁迫的植物中[内生真菌名称]的新靶基因，并突出了内生真菌对面临较少研究的环境威胁的植物的益处。
本文的在线版本（10.1007/s12298-020-00922-y）包含补充材料，授权用户可获取。
