School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, UK.
Nat Commun. 2023 Jul 10;14(1):4065. doi: 10.1038/s41467-023-39885-5.
Plants have benefited from interactions with symbionts for coping with challenging environments since the colonisation of land. The mechanisms of symbiont-mediated beneficial effects and similarities and differences to pathogen strategies are mostly unknown. Here, we use 106 (effector-) proteins, secreted by the symbiont Serendipita indica (Si) to modulate host physiology, to map interactions with Arabidopsis thaliana host proteins. Using integrative network analysis, we show significant convergence on target-proteins shared with pathogens and exclusive targeting of Arabidopsis proteins in the phytohormone signalling network. Functional in planta screening and phenotyping of Si effectors and interacting proteins reveals previously unknown hormone functions of Arabidopsis proteins and direct beneficial activities mediated by effectors in Arabidopsis. Thus, symbionts and pathogens target a shared molecular microbe-host interface. At the same time Si effectors specifically target the plant hormone network and constitute a powerful resource for elucidating the signalling network function and boosting plant productivity.
自陆地殖民以来，植物通过与共生体的相互作用，从中受益，以应对具有挑战性的环境。共生体介导的有益效应的机制以及与病原体策略的相似性和差异性在很大程度上尚不清楚。在这里，我们使用了 106 种（效应子）蛋白质，这些蛋白质由共生体 Serendipita indica（Si）分泌，以调节宿主生理学，从而与拟南芥宿主蛋白进行映射相互作用。通过整合网络分析，我们显示出与病原体共享的靶蛋白和在植物激素信号网络中拟南芥蛋白的特异性靶向之间存在显著的趋同。Si 效应子和相互作用蛋白的功能在体内筛选和表型分析揭示了拟南芥蛋白的先前未知激素功能以及效应子在拟南芥中介导的直接有益活性。因此，共生体和病原体针对共享的分子微生物-宿主界面。同时，Si 效应子特异性靶向植物激素网络，构成了阐明信号网络功能和提高植物生产力的强大资源。
