Schiøler V, Thode J
Department of Clinical Chemistry, Herlev University Hospital, Denmark.
Clin Chem. 1988 May;34(5):949-52.
We evaluated the analytical performance of six commercial direct radioimmunoassays of 17 beta-estradiol, those from Radioassay Systems Laboratories, IRE Medgenix, Biotecx Laboratories, Farmos Spectria, International CIS, and Diagnostic Products Corp. The mean value for estradiol (E2) and the within- and between-run CVs were determined for serum pools and control materials, measured in seven to 10 runs with each method. Mean values for E2 in pooled sera deviated by 75% to 350% from the means measured with our routinely used extraction method. Between-run CVs ranged from 4% to 14% for the direct assays as compared with 10% for the extraction assay. We also investigated, for two of the direct assays, the effect of extraction with diethyl ether before radioimmunoassay, with respect to improvement in the agreement with our extraction method. All of the assays were easy to perform and results were obtained within 4-5 h, but we conclude that matrix effects may be expected in direct assays of estradiol.
我们评估了六种市售的17β - 雌二醇直接放射免疫测定法的分析性能，这些方法分别来自放射分析系统实验室、IRE Medgenix、Biotecx实验室、Farmos Spectria、国际CIS和诊断产品公司。对血清池和对照材料测定了雌二醇（E2）的平均值以及批内和批间变异系数（CV），每种方法进行7至10次测定。混合血清中E2的平均值与我们常规使用的提取方法测得的平均值相比偏差了75%至350%。直接测定法的批间CV范围为4%至14%，而提取测定法为10%。我们还针对其中两种直接测定法，研究了在放射免疫测定前用乙醚提取的效果，以提高与我们提取方法的一致性。所有测定法操作简便，4至5小时内即可获得结果，但我们得出结论，在雌二醇的直接测定中可能会出现基质效应。
