Meleagros L, Ghatei M A, Anderson J V, Wharton J, Taylor K M, Krikler D M, Meijler F L, Polak J M, Bloom S R
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Clin Chim Acta. 1988 Mar 15;172(2-3):199-209. doi: 10.1016/0009-8981(88)90324-5.
A sensitive and specific radioimmunoassay has been developed for cardiodilatin, the N-terminal peptide sequence of the atrial natriuretic peptide (ANP) prohormone. Cardiodilatin-immunoreactivity (-IR) concentrations in the human right atrial appendage were found to correlate with ANP-IR concentrations, determined by an established radioimmunoassay, (cardiodilatin-IR = 13.2 +/- 1.2 nmol/g, ANP-IR = 19.8 +/- 2.0 nmol/g, r = 0.80, p less than 0.001). Characterisation of the cardiodilatin-IR in the human and rat right atrium by gel permeation and fast protein liquid chromatography revealed only two cardiodilatin-IR molecular forms. The larger more hydrophobic form, the majority of the cardiodilatin-IR, contained in addition ANP-IR and therefore represents the prohormone. The smaller, less hydrophobic form, lacked ANP-IR and thus represents the cleaved N-terminal peptide sequence of the prohormone. These findings indicate that the prohormone is the major molecular form in the human and rat atrium. Furthermore, they demonstrate that a single large N-terminal peptide, cardiodilatin, derived from the prohormone, may exist as a distinct molecular form in the atrium of these species.
已开发出一种针对心钠素（心房利钠肽原激素的N端肽序列）的灵敏且特异的放射免疫分析法。通过已建立的放射免疫分析法测定，发现人右心耳中心钠素免疫反应性（-IR）浓度与心钠素-IR浓度相关（心钠素-IR = 13.2±1.2 nmol/g，心钠素-IR = 19.8±2.0 nmol/g，r = 0.80，p＜0.001）。通过凝胶渗透和快速蛋白质液相色谱法对人和大鼠右心房中心钠素-IR进行表征，结果显示只有两种心钠素-IR分子形式。较大且疏水性更强的形式，即大部分心钠素-IR，还包含心钠素-IR，因此代表前激素。较小且疏水性较弱的形式缺乏心钠素-IR，因此代表前激素经切割后的N端肽序列。这些发现表明前激素是人和大鼠心房中的主要分子形式。此外，它们证明源自前激素的单一大型N端肽心钠素可能在这些物种的心房中以独特的分子形式存在。
