Morphometrical and Molecular Characterization of (Chabertiidae: Oesophagostominae) and (Trichostrongylidae: Haemonchinae) Isolated from Goat () in Sylhet, Bangladesh.

This study was aimed at describing two (2) intestinal nematodes from naturally infected native breed of goats () in Bangladesh, identified as (Curtice, 1890) Stossich 1899 and (Rudolphi, 1803) Cobb, 1898. The identification was made based on morphometric features and was confirmed by amplifying internal transcribed spacer (ITS) and cytochrome oxidase (1) gene. Well-developed lateral alae, distinct cervical papillae anteriorly to esophageal expansion, and male spicule length (0.73-0.79 mm, = 2) were characteristically observed in . At the same time, male spicule length (0.40-0.46 mm, = 2) and position of female vulvar flap (4.30-4.54 mm from posterior end, = 3) were observed in . DNA sequence homology of the ITS and 1 gene of both specimens revealed the same results, showing similarity to the GenBank sequences of (GenBank No. KC715827; JX188470) and (GenBank No. KJ724377; HQ389229). Phylogenetic analysis computed by maximum livelihood (ML) from the ITS nucleotide sequences revealed that the and isolates identified in this study were clustered in the same clade with isolates from China and Iran, respectively. This study, for the first time, illustrates the characteristics of and in Bangladesh, combining both morphological and molecular data. The universal primer-based polymerase chain reaction (PCR) protocol could be an economical and efficient option for researchers from poor resource settings for precise identification of nematodes. The information generated in this study may contribute to formulating effective control strategies against these nematodes.