Das B, Kumar N, Solanki J B, Jadav M M, Kalyani I H
Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396 450, Gujarat, India.
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari-396 450, Gujarat, India.
Helminthologia. 2023 Sep 22;60(2):175-188. doi: 10.2478/helm-2023-0020. eCollection 2023 Jun.
The successful design of strategic control measures against the blood-sucking gastrointestinal nematode, in small ruminants can be facilitated by revealing its general features from morphology to the molecular level. In the south Gujarat region of India, a total of 2408 were collected from 84 slaughtered sheep's abomasum, consisting of 347 males and 2061 females (1:6 ratio) (p<0.05). Furthermore, 726 were collected from 61 goats, comprising 145 males and 581 females (1:4 ratio) (p<0.05). The male worms were approximately 12±0.06 mm long, while female worms were about 20±0.09 mm long. The vulvar morphotypes of the female worms were found to be 17.7% linguiform, 76.6 % knobbed/button (p<0.05), and 5.7 % smooth type, demonstrating common features of . The nucleotide sequences of the Internal Transcribed Spacer 1 (ITS-1) of 165 bp or ITS-2 plus of 256 bp were aligned, and it was found that the genotypes of male and female specimens of either sheep or goat origin were identical, with a 100 % match. The present isolates shared >95 % and >94 % homology with published sequences of ITS-1 and ITS-2 plus of , respectively, with more nucleotide transitions than transversions in the aligned sequences. The reconstructed phylogram of either ITS-1 or ITS-2 plus revealed two major clades, one for and another for other nematodes, with showing its proximity with the clade of . The study established the role of morphological and molecular features in identifying and differentiating parasite at the local level.
通过揭示吸血性胃肠线虫从小形态学水平到分子水平的一般特征，有助于成功设计针对小型反刍动物的战略控制措施。在印度古吉拉特邦南部，从84只屠宰绵羊的皱胃中总共收集到2408条线虫，其中包括347只雄虫和2061只雌虫（比例为1:6）（p<0.05）。此外，从61只山羊中收集到726条线虫，包括145只雄虫和581只雌虫（比例为1:4）（p<0.05）。雄虫体长约为12±0.06毫米，雌虫体长约为20±0.09毫米。发现雌虫的阴门形态类型为17.7%舌状、76.6%有瘤/纽扣状（p<0.05）和5.7%光滑型，显示出[该线虫名称未给出]的共同特征。对165 bp的内转录间隔区1（ITS-1）或256 bp的ITS-2加序列的核苷酸序列进行比对，发现绵羊或山羊来源的雄虫和雌虫标本的基因型相同，匹配率为100%。目前的分离株与已发表的[该线虫名称未给出]的ITS-1和ITS-2加序列分别具有>95%和>94%的同源性，比对序列中的核苷酸转换多于颠换。重建的ITS-1或ITS-2加系统发育树显示有两个主要分支，一个是[该线虫名称未给出]的，另一个是其他线虫的，[该线虫名称未给出]显示出与[另一线虫名称未给出]分支的亲缘关系。该研究确定了形态学和分子特征在本地识别和区分[该线虫名称未给出]寄生虫中的作用。
