Sharma Gunjan, Boby Elza, Nidhi Thakur, Jain Ayushi, Singh Jay, Singh Archna, Chattopadhyay Parthaprasad, Bakhshi Sameer, Chopra Anita, Palanichamy Jayanth Kumar
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Department of Laboratory Oncology, Dr. B.R. Ambedkar Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India.
Front Oncol. 2021 Feb 23;11:588101. doi: 10.3389/fonc.2021.588101. eCollection 2021.
Around 85% of childhood Acute Lymphoblastic Leukemia (ALL) are of B-cell origin and characterized by the presence of different translocations including , , , and fusion proteins. The current clinical investigations used to identify translocation include FISH and fusion transcript specific PCR. In the current study we assessed the utility of , an oncofetal RNA binding protein, that is over expressed specifically in translocation positive B-ALL to be used as a diagnostic marker in the clinic. Further, public transcriptomic and Crosslinked Immunoprecipitation (CLIP) datasets were analyzed to identify the putative targets of IGF2BP1. We also studied the utility of using the mRNA expression of two such targets, and as potential diagnostic markers separately or in conjunction with . We observed that the expression of alone measured by RT-qPCR is highly sensitive and specific to be used as a potential biomarker for the presence of translocation in future.
约85%的儿童急性淋巴细胞白血病（ALL）起源于B细胞，其特征是存在不同的易位，包括 、 、 和 融合蛋白。目前用于鉴定 易位的临床研究方法包括荧光原位杂交（FISH）和融合转录本特异性聚合酶链反应（PCR）。在本研究中，我们评估了一种癌胚RNA结合蛋白 的效用，该蛋白在 易位阳性的B-ALL中特异性过表达，有望用作临床诊断标志物。此外，我们分析了公开的转录组学和交联免疫沉淀（CLIP）数据集，以确定胰岛素样生长因子2结合蛋白1（IGF2BP1）的推定靶点。我们还研究了单独或与 联合使用两个此类靶点（ 和 ）的mRNA表达作为潜在诊断标志物的效用。我们观察到，通过逆转录定量PCR（RT-qPCR）单独检测 的表达，对于未来作为 易位存在的潜在生物标志物具有高度敏感性和特异性。
