Department of Hematology, Dazhou Central Hospital, Tongchuan District, 56 Nanyue Temple Street Sichuan, Dazhou, 635000, China.
Ir J Med Sci. 2022 Oct;191(5):1995-2001. doi: 10.1007/s11845-021-02781-2. Epub 2021 Oct 11.
We aimed to investigate the relationship among epidermal growth factor-like protein-7 (EGFL7), integrin subunit beta 3 (ITGB3), and Kruppel-like factor 2 (KLF2) expressions and their clinical implication in multiple myeloma (MM).
This prospective study enrolled 72 de novo symptomatic MM patients and 30 controls, and then collected their bone marrow plasma cell samples. Subsequently, the EGFL7, ITGB3, and KLF2 expressions were carried out by reverse transcription quantitative polymerase chain reaction.
EGFL7, ITGB3, and KLF2 expressions were increased in MM patients compared to controls. Besides, EGFL7, ITGB3, and KLF2 inter-correlated with each other in MM patients but not in controls. In MM patients, EGFL7 and ITGB3 (but not KLF2) expressions were positively correlated with ISS stage, while ITGB3 and KLF2 (but not EGFL7) expressions were correlated with increased R-ISS stage. Interestingly, ITGB3 and KLF2 were decreased in induction-treatment complete remission (CR) MM patients compared to non-CR MM patients, while EGFL7 only showed a trend but without statistical significance. Furthermore, ITGB3 high expression was correlated with worse progression-free survival (PFS) and overall survival (OS), while EGFL7 and KLF2 high expressions only associated with pejorative PFS but not OS.
EGFL7, ITGB3, and KLF2 may serve as potential prognostic indicators in MM patients.
本研究旨在探讨表皮生长因子样蛋白 7（EGFL7）、整合素亚基β3（ITGB3）和 Kruppel 样因子 2（KLF2）的表达与多发性骨髓瘤（MM）之间的关系及其临床意义。
前瞻性纳入 72 例初治有症状 MM 患者和 30 例对照，收集骨髓浆细胞样本。采用逆转录定量聚合酶链反应检测 EGFL7、ITGB3 和 KLF2 的表达。
与对照组相比，MM 患者的 EGFL7、ITGB3 和 KLF2 表达均升高。此外，MM 患者中 EGFL7、ITGB3 和 KLF2 相互之间存在相关性，但在对照组中则没有。在 MM 患者中，EGFL7 和 ITGB3（但不是 KLF2）的表达与 ISS 分期呈正相关，而 ITGB3 和 KLF2（但不是 EGFL7）的表达与增加的 R-ISS 分期相关。有趣的是，与非完全缓解（CR）MM 患者相比，诱导治疗后 CR MM 患者的 ITGB3 和 KLF2 表达降低，而 EGFL7 仅表现出趋势但无统计学意义。此外，ITGB3 高表达与无进展生存期（PFS）和总生存期（OS）较差相关，而 EGFL7 和 KLF2 高表达仅与 PFS 预后不良相关，而与 OS 无关。
EGFL7、ITGB3 和 KLF2 可能作为 MM 患者的潜在预后指标。
