Mao Yuqing, Zeineldin Mohamed, Usmani Moiz, Uprety Sital, Shisler Joanna L, Jutla Antarpreet, Unnikrishnan Avinash, Nguyen Thanh H
Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign Urbana IL USA.
Institute for Genomic Biology University of Illinois at Urbana-Champaign Urbana IL USA.
Geohealth. 2021 Feb 1;5(2):e2020GH000294. doi: 10.1029/2020GH000294. eCollection 2021 Feb.
In this study, water samples were analyzed from a rural area of North Carolina after Hurricane Florence in 2018 and the distribution of the virulence gene of were investigated. We also examined the distribution of culturable and determined their antibiotic resistance profiles. Antibiotic resistance genes (ARGs) in the classes of aminoglycoside, beta-lactam, and macrolide-lincosamide-streptogramin B (MLSB) were targeted in this study. The gene was detected in 23 out of 25 locations. There was a wider and higher range of the gene in flooded water versus unflooded water samples (0-2.12 × 10 copies/L vs. 0-4.86 × 10 copies/L). Culturable was isolated from 10 of 25 sampling locations, which was less prevalent than the distribution of the gene. The antibiotic resistance profiles were not distinct among the isolates. The aminoglycoside resistance gene had the highest relative abundance (around 0.05 copies/16S rRNA gene copy in all isolates) among all ARGs. These findings suggested that the 2018 flooding event led to higher copy numbers of the genes of in some flooded water bodies compared to those in unflooded water bodies. The high ARG level and similar ARG profiles were observed in all isolates from both flooded and unflooded samples, suggesting that the antibiotic resistance was prevalent in within this region, regardless of flooding.
在本研究中，对2018年飓风“佛罗伦萨”过后北卡罗来纳州一个农村地区的水样进行了分析，并调查了弧菌毒力基因的分布情况。我们还检测了可培养弧菌的分布，并确定了它们的抗生素抗性谱。本研究针对氨基糖苷类、β-内酰胺类和大环内酯-林可酰胺-链阳霉素B（MLSB）类中的抗生素抗性基因（ARGs）。在25个地点中的23个检测到了该基因。与未受洪水影响的水样相比，受洪水影响的水样中该基因的范围更广、数量更多（0 - 2.12×10拷贝/升 vs. 0 - 4.86×10拷贝/升）。从25个采样地点中的10个分离出了可培养弧菌，其流行程度低于该基因的分布。弧菌分离株的抗生素抗性谱没有明显差异。在所有ARGs中，氨基糖苷抗性基因aadA在所有分离株中的相对丰度最高（约0.05拷贝/16S rRNA基因拷贝）。这些发现表明，与未受洪水影响的水体相比，2018年的洪水事件导致一些受洪水影响的水体中弧菌的该基因拷贝数更高。在受洪水影响和未受洪水影响样本中的所有弧菌分离株中均观察到高ARG水平和相似的ARG谱，这表明无论是否发生洪水，该地区的弧菌中抗生素抗性都很普遍。
