Buddhasiri Songphon, Sukjoi Chutikarn, Kaewsakhorn Thattawan, Nambunmee Kowit, Nakphaichit Massalin, Nitisinprasert Sunee, Thiennimitr Parameth
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
Front Microbiol. 2021 Aug 23;12:716761. doi: 10.3389/fmicb.2021.716761. eCollection 2021.
Acute non-typhoidal salmonellosis (NTS) caused by Typhimurium (STM) is among the most prevalent of foodborne diseases. A global rising of antibiotic resistance strains of STM raises an urgent need for alternative methods to control this important pathogen. Major human food animals which harbor STM in their gut are cattle, swine, and poultry. Previous studies showed that the probiotic () KUB-AC5 (AC5) exhibited anti- activities in chicken by modulating gut microbiota and the immune response. However, the immunobiotic effect of AC5 in a mammalian host is still not known. Here, we investigated the anti- and anti-inflammatory effects of AC5 on STM infection using a mouse colitis model. Three groups of C57BL/6 mice (prophylactic, therapeutic, and combined) were fed with 10 colony-forming units (cfu) AC5 daily for 7, 4, and 11 days, respectively. Then, the mice were challenged with STM compared to the untreated group. By using a specific primer pair, we found that AC5 can transiently colonize mouse gut (colon, cecum, and ileum). Interestingly, AC5 reduced STM gut proliferation and invasion together with attenuated gut inflammation and systemic dissemination in mice. The decreased STM numbers in mouse gut lumen, gut tissues, and spleen possibly came from longer AC5 feeding duration and/or the combinatorial (direct and indirect inhibitory) effect of AC5 on STM. However, AC5 attenuated inflammation (both in the gut and in the spleen) with no difference between these three approaches. This study demonstrated that AC5 confers both direct and indirect inhibitory effects on STM in the inflamed gut.
由鼠伤寒沙门氏菌（STM）引起的急性非伤寒型沙门氏菌病（NTS）是最常见的食源性疾病之一。全球范围内STM抗生素耐药菌株的增加迫切需要替代方法来控制这种重要的病原体。肠道中携带STM的主要人类食用动物是牛、猪和家禽。先前的研究表明，益生菌KUB-AC5（AC5）通过调节肠道微生物群和免疫反应在鸡中表现出抗（）活性。然而，AC5在哺乳动物宿主中的免疫生物效应仍然未知。在这里，我们使用小鼠结肠炎模型研究了AC5对STM感染的抗菌和抗炎作用。三组C57BL/6小鼠（预防组、治疗组和联合组）分别每天喂食10个菌落形成单位（cfu）的AC5，持续7天、4天和11天。然后，与未治疗组相比，用STM对小鼠进行攻击。通过使用特定的引物对，我们发现AC5可以短暂地定殖于小鼠肠道（结肠、盲肠和回肠）。有趣的是，AC5减少了STM在肠道中的增殖和侵袭，同时减轻了小鼠的肠道炎症和全身扩散。小鼠肠腔、肠道组织和脾脏中STM数量的减少可能来自于AC5更长的喂食时间和/或AC5对STM的组合（直接和间接抑制）作用。然而，AC5减轻了炎症（在肠道和脾脏中），这三种方法之间没有差异。这项研究表明，AC5在炎症肠道中对STM具有直接和间接的抑制作用。
