Department of Biotechnology and Crop Improvement, College of Horticulture, Bagalkot, University of Horticultural Sciences, Bagalkot, India.
Directorate of Research, University of Horticultural Sciences, Bagalkot, India.
Mol Plant Microbe Interact. 2021 Jul;34(7):874-877. doi: 10.1094/MPMI-01-21-0001-A. Epub 2021 Aug 24.
pv. causing bacterial blight is a devastating disease of pomegranate in India and Pakistan. Most xanthomonads use the type III secretion system to inject transcription activator-like effector (TALE) proteins into the host cell. TALEs bind to the effector-binding elements in the promoter of host susceptibility genes, triggering disease development. PacBio single-molecule real-time long-read sequencing technology was used to identify the TALE-encoding genes, which is otherwise not possible using next-generation short-read sequencers. In all, 1.74 Gb of raw data containing 368,980 subreads, with an average read length of 4,724 bp and longest read length of 77,471, were generated. Subreads were assembled into 15 scaffolds generating approximately 5.4 Mb (348×) of genome. pv. exhibited close lineage with pv. with 98.78% average nucleotide identity. Of the 4,263 protein-coding genes, 11 non-TALE type III effectors and 2 TALE-encoding genes were identified.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
pv. 引起细菌性枯萎病是石榴在印度和巴基斯坦的一种毁灭性病害。大多数黄单胞菌利用 III 型分泌系统将转录激活因子样效应蛋白（TALE）注入宿主细胞。TALEs 结合到宿主易感性基因启动子中的效应结合元件上，触发疾病的发展。PacBio 单分子实时长读测序技术被用于鉴定 TALE 编码基因，这在使用下一代短读测序仪时是不可能的。总共生成了 1.74GB 的原始数据，包含 368980 个亚读长，平均读长为 4724bp，最长读长为 77471bp。亚读长组装成 15 个支架，生成约 5.4Mb（348×）的基因组。pv. 与 pv. 具有 98.78%的平均核苷酸同一性，表现出密切的亲缘关系。在 4263 个蛋白质编码基因中，鉴定出 11 个非 TALE III 型效应物和 2 个 TALE 编码基因。[公式：见正文]版权所有 © 2021 作者。这是一个 CC BY-NC-ND 4.0 国际许可下的开放获取文章。
