Faculty of Medicine, Mahasarakham University, Maha Sarakham, 44000, Thailand.
Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Eur J Pharmacol. 2021 May 15;899:174010. doi: 10.1016/j.ejphar.2021.174010. Epub 2021 Mar 9.
In the present study, the therapeutic effects of imperatorin on metabolic and vascular alterations and possible underlying mechanisms were investigated in high-fat/high-fructose diet (HFFD)-fed rats. Male Sprague-Dawley rats were fed a high-fat diet plus 15% fructose in drinking water for 16 weeks. HFFD-fed rats were treated with imperatorin (15 or 30 mg/kg/day) for the last 4 weeks. In HFFD-fed rats, imperatorin significantly reduced obesity, hypertension, dyslipidemia, and insulin resistance. Imperatorin markedly improved vascular endothelial function and alleviated changes in vascular morphology. Furthermore, imperatorin treatment significantly increased the plasma levels of the nitric oxide metabolite and adiponectin, and upregulated adiponectin receptor 1 and endothelial nitric oxide synthase (eNOS) protein expression in the thoracic aorta. Imperatorin treatment decreased vascular superoxide anion production and downregulated aortic NADPH oxidase subunit p47 protein expression. These findings indicated that imperatorin alleviates HFFD-induced metabolic and vascular alterations in rats. The possible underlying mechanism may involve the restoration of adiponectin receptor 1 and eNOS expression and suppression of p47 expression.
在本研究中，我们研究了大茴香脑对高脂高果糖饮食（HFFD）喂养大鼠代谢和血管改变的治疗作用及其可能的机制。雄性 Sprague-Dawley 大鼠给予高脂肪饮食加饮用水中 15%的果糖喂养 16 周。HFFD 喂养的大鼠在最后 4 周给予大茴香脑（15 或 30mg/kg/天）治疗。在 HFFD 喂养的大鼠中，大茴香脑显著减轻肥胖、高血压、血脂异常和胰岛素抵抗。大茴香脑显著改善血管内皮功能并减轻血管形态变化。此外，大茴香脑治疗显著增加血浆一氧化氮代谢物和脂联素水平，并上调胸主动脉中的脂联素受体 1 和内皮型一氧化氮合酶（eNOS）蛋白表达。大茴香脑治疗减少血管超氧阴离子产生并下调主动脉 NADPH 氧化酶亚基 p47 蛋白表达。这些发现表明大茴香脑可减轻 HFFD 诱导的大鼠代谢和血管改变。可能的机制涉及恢复脂联素受体 1 和 eNOS 表达并抑制 p47 表达。
