Department of Veterinary Medicine, Konkuk University, Seoul, 05029, Republic of Korea.
GeneReach Biotechnology, Taichung, Taiwan, ROC.
J Virol Methods. 2021 Jun;292:114126. doi: 10.1016/j.jviromet.2021.114126. Epub 2021 Mar 9.
Highly pathogenic avian influenza viruses (HPAIVs) and low pathogenic avian influenza viruses (LPAIVs) represent important threats to the poultry industry and global human health. Due to the high rates of avian influenza virus (AIV) transmission, controlling AIV outbreaks is challenging. HPAIV is known to be transmitted from wild birds to domestic ducks, from which it can be transmitted to layer and broiler chickens. Therefore, surveillance of AIV in domestic ducks and chickens in advance of outbreaks can prevent its spread and enable timely implementation of disease control measures. Certain molecular diagnostic tools can be applied in the field for faster AIV detection. In this study, we evaluated the AIV-detection ability of two insulated isothermal PCR (iiPCR) devices, POCKIT™Micro DUO Nucleic Acid Analyzer (POCKIT DUO) and POCKIT™ Central Nucleic Acid Analyzer (POCKIT Central). We found that the analytical, in vivo and clinical performances of the two POCKIT devices were comparable to those of real-time reverse transcription PCR. Due to their brief protocols and short detection times, POCKIT DUO and POCKIT Central represent promising molecular diagnostic devices for the reliable detection of AIV.
高致病性禽流感病毒（HPAIVs）和低致病性禽流感病毒（LPAIVs）对家禽业和全球人类健康构成重大威胁。由于禽流感病毒（AIV）的高传播率，控制 AIV 爆发具有挑战性。已知 HPAIV 可从野鸟传播到家养鸭，然后从鸭传播到蛋鸡和肉鸡。因此，在爆发前对家养鸭和鸡中的 AIV 进行监测可以防止其传播，并能够及时实施疾病控制措施。某些分子诊断工具可用于现场进行更快的 AIV 检测。在这项研究中，我们评估了两种隔热等温 PCR（iiPCR）设备 POCKIT™Micro DUO Nucleic Acid Analyzer（POCKIT DUO）和 POCKIT™Central Nucleic Acid Analyzer（POCKIT Central）的 AIV 检测能力。我们发现，两种 POCKIT 设备的分析性能、体内性能和临床性能均与实时逆转录 PCR 相当。由于其简短的方案和较短的检测时间，POCKIT DUO 和 POCKIT Central 是用于可靠检测 AIV 的有前途的分子诊断设备。
