Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
Semin Thorac Cardiovasc Surg. 2022 Spring;34(1):349-358. doi: 10.1053/j.semtcvs.2021.03.010. Epub 2021 Mar 10.
Bronchopleural fistula is one of the most serious postoperative complications caused by the incomplete healing of a bronchial stump. Fibroblasts play an important role in wound healing by facilitating connective tissue formation and inducing angiogenesis. We developed a method for production of multilayered fibroblast sheets that secreted some growth factors and promoted wound healing. The present study aimed to assess the treatment effect of multilayered fibroblast sheets on bronchial stump healing. In this rat model, left pneumonectomy was performed, and multilayered fibroblast sheets derived from autologous oral mucosal tissues were transplanted to the bronchial stump. The changes in the bronchial stump were examined macroscopically, histologically, and mechanically. The fibroblast sheets promoted the formation of thick connective tissues around the bronchial stump. The formed connective tissues were accompanied by new blood vessels, and fibrosis was observed over time. Then, 7 days after the transplantation of the fibroblast sheets, the bronchial wall became significantly thicker, and the area of the blood vessels for the bronchial wall tissues was significantly larger in the experimental group than in the control group. In addition, the burst pressure in the bronchial stump was significantly higher in the experimental group than in the control group. Bronchial stumps were reinforced by the transplantation of multilayered fibroblast sheets derived from autologous oral mucosal tissues.
支气管胸膜瘘是支气管残端愈合不完全引起的最严重的术后并发症之一。成纤维细胞通过促进结缔组织形成和诱导血管生成，在伤口愈合中起着重要作用。我们开发了一种生产多层成纤维细胞片的方法，这些细胞片分泌一些生长因子并促进伤口愈合。本研究旨在评估多层成纤维细胞片对支气管残端愈合的治疗效果。在这个大鼠模型中，进行左全肺切除术，并将源自自体口腔黏膜组织的多层成纤维细胞片移植到支气管残端。宏观、组织学和力学检查支气管残端的变化。成纤维细胞片促进了支气管残端周围厚结缔组织的形成。形成的结缔组织伴随着新的血管，随着时间的推移会出现纤维化。然后，在移植成纤维细胞片 7 天后，实验组的支气管壁明显变厚，支气管壁组织的血管面积明显大于对照组。此外，实验组的支气管残端爆裂压明显高于对照组。支气管残端通过移植源自自体口腔黏膜组织的多层成纤维细胞片得到加强。
