同种异体多层成纤维细胞片促进大鼠食管重建模型吻合口愈合。

Allogenic multilayered fibroblast sheets promote anastomotic site healing in a rat model of esophageal reconstruction.

作者信息

Yamamoto Naohiro, Ueno Koji, Yanagihara Masashi, Kurazumi Hiroshi, Tanaka Yuya, Oga Atsunori, Shimokawa Mototsugu, Harada Eijiro, Tanaka Toshiki, Hamano Kimikazu

机构信息

Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine Ube, Yamaguchi, Japan.

Department of Molecular Pathology, Yamaguchi University Graduate School of Medicine Ube, Yamaguchi, Japan.

出版信息

Am J Transl Res. 2023 May 15;15(5):3217-3228. eCollection 2023.

PMID:37303629

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10250989/

Abstract

OBJECTIVE

Anastomotic leakage is a common and severe complication of esophageal reconstruction. Accordingly, there is a clinical need for novel methods to prevent it. We developed multilayered, growth factor-secreting fibroblast sheets that promote wound healing and angiogenesis. The present study aimed to assess the utility of allogenic multilayered fibroblast sheets in preventing esophageal anastomotic leakage in a rat model of esophageal reconstruction.

METHODS

Allogenic multilayered fibroblast sheets prepared from oral mucosal tissues were implanted at esophageal anastomotic sites.

RESULTS

The allogenic multilayered fibroblast sheet group had significantly higher burst pressure and collagen deposition compared to a control group five days postoperatively. The expression levels of collagen type I and III mRNAs around esophageal suture sites were higher in the allogenic multilayered fibroblast sheet group compared to the control group on postoperative days 0, 3, and 5. There was a trend toward lower anastomotic leakage and lower abscess scores in the allogenic multilayered fibroblast sheet group compared to the control group; however, these differences did not reach statistical significance. Allogenic multilayered fibroblast sheets completely disappeared at ten days after implantation. Further, no inflammation was observed at suture sites with implanted allogenic multilayered fibroblast sheets at five days after surgery.

CONCLUSION

Allogenic multilayered fibroblast sheets may represent a promising method of preventing esophageal anastomotic leakage.

摘要

目的

吻合口漏是食管重建常见且严重的并发症。因此，临床上需要新的预防方法。我们研发了可分泌生长因子的多层成纤维细胞片，其能促进伤口愈合和血管生成。本研究旨在评估同种异体多层成纤维细胞片在大鼠食管重建模型中预防食管吻合口漏的效用。

方法

将由口腔黏膜组织制备的同种异体多层成纤维细胞片植入食管吻合部位。

结果

术后5天，与对照组相比，同种异体多层成纤维细胞片组的破裂压力和胶原蛋白沉积显著更高。术后0、3和5天，同种异体多层成纤维细胞片组食管缝合部位周围I型和III型胶原蛋白mRNA的表达水平高于对照组。与对照组相比，同种异体多层成纤维细胞片组有吻合口漏减少和脓肿评分降低的趋势；然而，这些差异未达到统计学意义。同种异体多层成纤维细胞片在植入后10天完全消失。此外，术后5天，在植入同种异体多层成纤维细胞片的缝合部位未观察到炎症。