Jiangsu Key Laboratory of Sericulture Biology and Biotechnology, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, PR China.
Jiangsu Key Laboratory of Sericulture Biology and Biotechnology, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, PR China.
Mar Pollut Bull. 2021 May;166:112222. doi: 10.1016/j.marpolbul.2021.112222. Epub 2021 Mar 9.
A marine diatom (Phaeodactylum tricornutum) was exposed to different concentrations of dimethyl phthalate (DMP) and diethyl phthalate (DEP) for 96 h within a batch-culture system to investigate their toxicities. Results showed that P. tricornutum could remove DMP and DEP effectively with removal rates of 0.20-0.30 and 0.14-0.21 mg L h, respectively. In addition, DMP and DEP significantly inhibited the photosynthesis and chlorophyll a biosynthesis of P. tricornutum with 96-h EC values of 390.5 mg L and 74.0 mg L, respectively. Results of reactive oxygen species (ROS) level suggested that the two PAEs could induce excessive ROS production in the diatom. Moreover, activities of antioxidant enzymes (i.e., SOD and POD) in the diatom increased with the increase of DMP and DEP concentrations. The results will help to understand the toxic mechanisms of PAEs, and provide strong evidences for evaluating their ecological risks in the marine environment.
采用批量培养系统，研究了海洋硅藻（Phaeodactylum tricornutum）在不同浓度邻苯二甲酸二甲酯（DMP）和邻苯二甲酸二乙酯（DEP）中的暴露 96 h 情况，以考察它们的毒性。结果表明，P. tricornutum 能够有效去除 DMP 和 DEP，去除率分别为 0.20-0.30 和 0.14-0.21 mg L h。此外，DMP 和 DEP 显著抑制了 P. tricornutum 的光合作用和叶绿素 a 合成，96 h 的 EC 值分别为 390.5 mg L 和 74.0 mg L。活性氧（ROS）水平的结果表明，这两种 PAEs 可诱导硅藻中过量的 ROS 产生。此外，随着 DMP 和 DEP 浓度的增加，硅藻中抗氧化酶（SOD 和 POD）的活性也随之增加。这些结果将有助于了解 PAEs 的毒性机制，并为评估它们在海洋环境中的生态风险提供有力证据。
