Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
National Research Tomsk Polytechnic University, Tomsk, Russia.
Bull Exp Biol Med. 2021 Feb;170(4):415-419. doi: 10.1007/s10517-021-05078-7. Epub 2021 Mar 13.
We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI18-35, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide-1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes Тс-DPAH-UBI29-41 and Тс-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography.
我们研究了一种新型放射性标记的抗菌肽 UBI29-41 和 UBI18-35 的复合制剂，即尿抑素衍生物，用于区分细菌性炎症和无菌性炎症。为了用锝-99m 对这些肽进行放射性标记，使用了双功能螯合剂琥珀酰亚胺基-1-基 6-（双（吡啶-2-基甲基）氨基）己酸。所得的配合物 Тс-DPAH-UBI29-41 和 Тс-DPAH-UBI18-35 的放射性标记产率>80%，放射化学纯度>96%。通过创伤性骨髓炎大鼠模型的单光子发射计算机断层扫描证实，该复合物在骨结构中的细菌性炎症病灶中的聚集，以及在无菌性炎症病灶中不存在该复合物。
