马尔堡病毒 DNA 疫苗的原型。

Prototype of a DNA Vaccine against Marburg Virus.

机构信息

State Research Centre of Virology and Biotechnology "Vector", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.

出版信息

Bull Exp Biol Med. 2021 Feb;170(4):475-478. doi: 10.1007/s10517-021-05091-w. Epub 2021 Mar 13.

DOI:10.1007/s10517-021-05091-w

PMID:33713231

Abstract

The preparation and study of the biological properties of the pVAKS-GPVM DNA immunogen containing a gene encoding Marburgvirus glycoprotein are described. The specificity of blood serum antibodies of guinea pigs immunized with DNA immunogen was analyzed by ELISA. Inactivated viral preparation, recombinant glycoprotein (GP) obtained in the prokaryotic system and virus-like particles based on the recombinant vesicular stomatitis virus exhibiting Marburgvirus GP were used as the antigens. The neutralizing activity of antibodies of immunized animals was tested in vitro using a pseudovirus system. It was demonstrated that the developed immunogen administered to guinea pigs induced the production of specific antibodies that neutralize virus-like particles and Marburgvirus in cultured Vero cells.

摘要

本文描述了含有编码马尔堡病毒糖蛋白基因的 pVAKS-GPVM DNA 免疫原的制备和生物学特性研究。通过 ELISA 分析了用 DNA 免疫原免疫的豚鼠血清抗体的特异性。使用作为抗原的灭活病毒制剂、在原核系统中获得的重组糖蛋白 (GP) 和基于展示马尔堡病毒 GP 的重组水疱性口炎病毒样颗粒来进行分析。在体外使用假病毒系统测试了免疫动物抗体的中和活性。结果表明，在豚鼠中使用的这种免疫原诱导产生了特异性抗体，该抗体可中和在培养的 Vero 细胞中的病毒样颗粒和马尔堡病毒。