Chair of Industrial Organic Chemistry and Biotechnology, Faculty of Chemistry, Bielefeld University, Universitätsstr. 25, 33615, Bielefeld, Germany.
Angew Chem Int Ed Engl. 2021 Jul 12;60(29):15863-15869. doi: 10.1002/anie.202015887. Epub 2021 Jun 10.
In modern process development, it is imperative to consider biocatalysis, and whole-cell catalysts often represent a favored form of such catalysts. However, the application of whole-cell catalysis in typical organic batch two-phase synthesis often struggles due to mass transfer limitations, emulsion formation, tedious work-up and, thus, low yields. Herein, we demonstrate that utilizing segmented flow tools enables the conduction of whole-cell biocatalysis efficiently in biphasic media. Exemplified for three different biotransformations, the power of such segmented flow processes is shown. For example, a 3-fold increase of conversion from 34 % to >99 % and a dramatic simplified work-up leading to a 1.5-fold higher yield from 44 % to 65 % compared to the analogous batch process was achieved in such a flow process.
在现代过程开发中，必须考虑生物催化，而全细胞催化剂通常代表此类催化剂的首选形式。然而，由于传质限制、乳液形成、繁琐的后处理等问题，全细胞催化在典型的有机分批两相合成中的应用往往受到阻碍，因此产率较低。在此，我们证明了利用分段流工具可以有效地在两相介质中进行全细胞生物催化。通过三种不同的生物转化实例证明了这种分段流过程的优势。例如，与类似的分批过程相比，转化率从 34%提高到>99%，工作流程大大简化，产率从 44%提高到 65%，提高了 1.5 倍。
