Tang Xiaoping, Allemann Rudolf K, Wirth Thomas
School of Chemistry Cardiff University Park Place, Main Building CF10 3AT Cardiff UK.
European J Org Chem. 2017 Jan 10;2017(2):414-418. doi: 10.1002/ejoc.201601388. Epub 2017 Jan 13.
Sesquiterpenes are an important family of natural products, many of which exhibit important pharmaceutical and agricultural properties. They are biosynthesised from farnesyl diphosphate in sesquiterpene synthase catalysed reactions. Here, we report the development of a highly efficient segmented flow system for the enzyme-catalysed continuous flow production of sesquiterpenes. Design of experiment (DoE) methods were used to optimise the performance of the flow biocatalysis, and quantitative yields were achieved by using an operationally simple but highly effective segmented flow system.
倍半萜是一类重要的天然产物，其中许多具有重要的医药和农业特性。它们是在倍半萜合酶催化的反应中由法尼基二磷酸生物合成的。在此，我们报告了一种高效的分段流动系统的开发，用于酶催化连续流动生产倍半萜。实验设计（DoE）方法用于优化流动生物催化的性能，通过使用操作简单但高效的分段流动系统实现了定量产率。
