Thy1 阳性精原细胞通过细胞外囊泡在体外抑制精原干细胞的增殖。
Thy1-Positive Spermatogonia Suppress the Proliferation of Spermatogonial Stem Cells by Extracellular Vesicles In Vitro.
机构信息
International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Speciality, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China.
出版信息
Endocrinology. 2021 Jul 1;162(7). doi: 10.1210/endocr/bqab052.
The self-renewal of mammalian spermatogonial stem cells (SSCs) supports spermatogenesis to produce spermatozoa, and this is precisely controlled in a stem niche microenvironment in the seminiferous tubules. Although studies have revealed the role of the surrounding factors in SSCs, little is known about whether the division of SSCs is controlled by extracellular vesicles. Here, extracellular vesicles were found in the basal compartment of seminiferous tubules in mouse, rat, rabbit and human testes. In the mice, the testicular extracellular vesicles are secreted by spermatogonia and are taken up by SSCs. Further, the extracellular vesicles from thy1-positive spermatogonia were purified by anti-Thy1-coupled magnetic beads, which suppress their proliferation of SSCs but do not lead to the apoptosis in vitro.
哺乳动物精原干细胞（SSC）的自我更新支持精子发生以产生精子，这正是在生精小管的干细胞龛微环境中精确控制的。尽管研究已经揭示了周围因素在 SSC 中的作用，但对于 SSC 的分裂是否受细胞外囊泡控制知之甚少。在这里，在小鼠、大鼠、兔子和人类睾丸的生精小管基底腔内发现了细胞外囊泡。在小鼠中，睾丸细胞外囊泡由精原细胞分泌，并被 SSC 摄取。此外，通过抗 Thy1 偶联磁珠从 Thy1 阳性精原细胞中纯化的细胞外囊泡可抑制 SSC 的增殖，但不会导致体外细胞凋亡。
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