QIMR Berghofer Medical Research Institute, Molecular Parasitology Laboratory, Brisbane, Australia; University of Queensland, Faculty of Medicine, Herston, 4006, QLD, Australia.
QIMR Berghofer Medical Research Institute, Molecular Parasitology Laboratory, Brisbane, Australia.
Exp Parasitol. 2021 May;224:108098. doi: 10.1016/j.exppara.2021.108098. Epub 2021 Mar 11.
Molecular diagnostics are powerful tools for disease detection but are typically confined to the laboratory environment due to the cumbersome methods required to extract nucleic acids from biological samples. Accurate diagnosis is essential for early detection of parasitic worm infections and for monitoring control programs, particularly during new transmission outbreaks to limit infection spread. We optimized the recently developed DNA dipstick technology to purify Schistosoma japonicum DNA from different life stages in <60 s. We successfully detected DNA from adult worms, eggs and infected snails. The speed and simplicity of this method enables the point-of-care detection of S. japonicum.
分子诊断是疾病检测的有力工具，但由于从生物样本中提取核酸所需的繁琐方法，通常仅限于实验室环境。准确的诊断对于寄生虫感染的早期检测以及监测控制计划至关重要，特别是在新的传播爆发期间，以限制感染的传播。我们优化了最近开发的 DNA 试纸条技术，使其能够在<60s 内从不同的生活阶段纯化日本血吸虫 DNA。我们成功地从成虫、虫卵和感染的蜗牛中检测到了 DNA。该方法的速度和简单性使得能够在现场检测日本血吸虫。
