Laboratorio de Inmunoparasitología (LAINPA), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional de La Plata (UNLP), La Plata, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Laboratorio de Inmunoparasitología (LAINPA), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional de La Plata (UNLP), La Plata, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Parasitol Int. 2021 Aug;83:102328. doi: 10.1016/j.parint.2021.102328. Epub 2021 Mar 10.
The allelic combination of ROP18/ROP5 genes of Toxoplasma gondii has been shown to be highly predictive of mouse virulence in canonical isolates and strains. The aims of this study were to analyze the alleles present in the ROP18/ROP5 genes from T. gondii isolates obtained in Argentina, to associate the results with the virulence registered in mouse model, and to compare with other isolates and reference strains using a phylogenetic network. Fourteen T. gondii isolates from Argentina were analyzed by nPCR-RFLP for ROP18/ROP5. Phylogenetic network analysis was inferred using the ToxoDB genotypes and the ROPs molecular markers. All isolates and reference strains were categorized as lethal or non-lethal. As results, combinations 2/2, 3/3 and 4/3 for ROP18/ROP5 were detected in 12 isolates, whereas only alleles 1 and 2 of ROP5 were detected in 2 isolates. The majority of the isolates had a mouse virulence matching to that predicted by the ROP18/ROP5 allele combination. The 3 isolates that differed from the expected virulence presented non-clonal genotypes. ROPs incorporation increased the accuracy of the phylogenetic network relations among the T. gondii samples, prevailing the clustering according to regions. Our results indicate a predominance of type 3 allele in both ROP18 and ROP5 markers and an association of allelic profiles 3/3 and 4/3 of non-clonal genotypes from Argentina, both with virulent and avirulent profiles in mice.
弓形虫 ROP18/ROP5 基因的等位基因组合已被证明在经典分离株和株中高度预测小鼠的毒力。本研究的目的是分析从阿根廷获得的弓形虫分离株中 ROP18/ROP5 基因的等位基因，将结果与小鼠模型中记录的毒力相关联，并与其他分离株和参考株使用系统发生网络进行比较。通过 nPCR-RFLP 对来自阿根廷的 14 个弓形虫分离株进行 ROP18/ROP5 分析。使用 ToxoDB 基因型和 ROPs 分子标记推断系统发生网络分析。所有分离株和参考株均被归类为致死或非致死。结果，在 12 个分离株中检测到 ROP18/ROP5 的组合 2/2、3/3 和 4/3，而在 2 个分离株中仅检测到 ROP5 的等位基因 1 和 2。大多数分离株的小鼠毒力与 ROP18/ROP5 等位基因组合预测的毒力相匹配。与预期毒力不同的 3 个分离株具有非克隆基因型。ROP 整合增加了弓形虫样本之间系统发生网络关系的准确性，根据地区聚类占主导地位。我们的结果表明，3 型等位基因在 ROP18 和 ROP5 标记中占优势，并且与来自阿根廷的非克隆基因型的 3/3 和 4/3 的等位基因谱相关联，在小鼠中均具有毒力和非毒力谱。
