Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, 25280 Saltillo, Coahuila, Mexico.
Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, 25280 Saltillo, Coahuila, Mexico.
Bioresour Technol. 2021 Jun;329:124935. doi: 10.1016/j.biortech.2021.124935. Epub 2021 Mar 6.
Sargassum spp is an invasive macroalgae and an alternative feedstock for bioethanol production. Sargassum spp biomass was subjected to high-pressure technology for biomass fractionation under different operating conditions of temperature and residence time to obtain glucan enriched pretreated solids (32.22 g/100 g of raw material). Enzyme hydrolysis process at high pretreated solid loading (13%, w/v) and enzyme loading of 10 FPU/g of glucan was performed, obtaining 43.01 g/L of glucose corresponding to a conversion yield of 92.12%. Finally, a pre-simultaneous saccharification and fermentation strategy (PSSF) was performed to produce bioethanol. This operational strategy produced 45.66 g/L of glucose in the pre-saccharification stage, and 18.14 g/L of bioethanol was produced with a glucose to bioethanol conversion yield of 76.23%. The development of this process highlights the feasibility of bioethanol production from macroalgal biomass in the biorefinery concept.
马尾藻属是一种入侵性大型藻类，也是生产生物乙醇的替代原料。马尾藻属生物质在不同温度和停留时间的操作条件下采用高压技术进行生物质分级，以获得富含葡聚糖的预处理固体（32.22 g/100 g 原料）。在高预处理固体负荷（13%，w/v）和 10 FPU/g 葡聚糖的酶水解过程中，得到了 43.01 g/L 的葡萄糖，转化率为 92.12%。最后，采用预同步糖化发酵策略（PSSF）生产生物乙醇。该操作策略在预糖化阶段产生了 45.66 g/L 的葡萄糖，在糖化阶段产生了 18.14 g/L 的生物乙醇，葡萄糖到生物乙醇的转化率为 76.23%。该工艺的开发突出了在生物炼制概念中从大型藻类生物质生产生物乙醇的可行性。
