Biorefinery Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo C.P. 25280, Coahuila, Mexico.
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Saltillo, Av. Industria Metalúrgica 1062, Ramos Arizpe C.P. 25900, Coahuila, Mexico.
Molecules. 2022 Jun 17;27(12):3887. doi: 10.3390/molecules27123887.
The development of green technologies and bioprocesses such as solid-state fermentation (SSF) is important for the processing of macroalgae biomass and to reduce the negative effect of spp. on marine ecosystems, as well as the production of compounds with high added value such as fungal proteins. In the present study, spp. biomass was subjected to hydrothermal pretreatments at different operating temperatures (150, 170, and 190 °C) and pressures (3.75, 6.91, and 11.54 bar) for 50 min, obtaining a glucan-rich substrate (17.99, 23.86, and 25.38 g/100 g d.w., respectively). The results indicate that pretreated at a pretreatment temperature of 170 °C was suitable for fungal growth. SSF was performed in packed-bed bioreactors, obtaining the highest protein content at 96 h (6.6%) and the lowest content at 72 h (4.6%). In contrast, it was observed that the production of fungal proteins is related to the concentration of sugars. Furthermore, fermentation results in a reduction in antinutritional elements, such as heavy metals (As, Cd, Pb, Hg, and Sn), and there is a decrease in ash content during fermentation kinetics. Finally, this work shows that can assimilate nutrients found in the pretreated spp. to produce fungal proteins as a strategy for the food industry.
开发绿色技术和生物工艺，如固态发酵（SSF），对于加工大型藻类生物质以及减少 spp. 对海洋生态系统的负面影响以及生产具有高附加值的化合物（如真菌蛋白）非常重要。在本研究中， spp. 生物质在不同操作温度（150、170 和 190°C）和压力（3.75、6.91 和 11.54 巴）下进行水热处理 50 分钟，得到富含葡聚糖的底物（分别为 17.99、23.86 和 25.38 g/100 g d.w.）。结果表明，在预处理温度为 170°C 时，预处理适合真菌生长。在填充床生物反应器中进行 SSF，在 96 小时时获得最高的蛋白质含量（6.6%），在 72 小时时获得最低的蛋白质含量（4.6%）。相比之下，观察到真菌蛋白的生产与糖的浓度有关。此外，发酵导致抗营养元素（如重金属（As、Cd、Pb、Hg 和 Sn））减少，并且在发酵动力学过程中灰分含量降低。最后，这项工作表明， 可以同化预处理 spp. 中发现的营养物质来生产真菌蛋白，作为食品工业的一种策略。
